Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Chemical reprogramming directly resets mouse post-implantation epiblast cells to a totipotent state.

Cell research·2026

Same author

Metabolic patterns predispose human pluripotent stem cells to spatial organization of cell fate.

Frontiers in cell and developmental biology·2026

Same author

Remodeling Cell Adhesion Releases Cardiac Potential of Human Pluripotent Stem Cells with Continuous Proliferation and Accelerated Maturation.

International journal of biological sciences·2025

Same author

A conserved function of EZH2-PRC2 in repression of primitive endoderm fate in mouse and human pluripotent cells.

Stem cell reports·2025

Same author

Editorial: Studying rare diseases using induced pluripotent stem cell (iPSC)-based model systems.

Frontiers in cell and developmental biology·2025

Same author

Modeling mammalian hibernation to improve organ cold preservation: Using the intestine as an example.

Cell research·2025

Search research articles

Related Experiment Video

Updated: Jan 3, 2026

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Published on: July 24, 2014

Developments in cell culture systems for human pluripotent stem cells.

Weiwei Liu¹, Chunhao Deng¹, Carlos Godoy-Parejo¹

¹Centre of Reproduction, Development and Aging, Faculty of Health Sciences, University of Macau, Macau 999078, China.

World Journal of Stem Cells

|November 27, 2019

Summary

Human pluripotent stem cells (hPSCs) are vital for therapies and drug development. Advances in hPSC culture technologies are crucial for unlocking their full potential in these applications.

Keywords:

Cell culture Culture medium Embryoid bodies Human embryonic stem cells Human pluripotent stem cells Signal transduction Stem cell niche

More Related Videos

Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

Published on: May 14, 2015

Author Spotlight: Automating iPSC Culture for Enhanced Reproducibility

Author Spotlight: Automating iPSC Culture for Enhanced Reproducibility

Published on: January 26, 2024

Related Experiment Videos

Last Updated: Jan 3, 2026

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Published on: July 24, 2014

Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

Published on: May 14, 2015

Author Spotlight: Automating iPSC Culture for Enhanced Reproducibility

Author Spotlight: Automating iPSC Culture for Enhanced Reproducibility

Published on: January 26, 2024

Area of Science:

Biotechnology
Stem Cell Biology
Regenerative Medicine

Background:

Human pluripotent stem cells (hPSCs) hold significant promise for cell-based therapies and pharmaceutical research.
Realizing the therapeutic potential of hPSCs necessitates the development of advanced, application-specific technologies.
Current hPSC technologies are heavily reliant on cell culture, with outcomes influenced by medium composition, extracellular matrices, handling, and culture platforms.

Purpose of the Study:

To review recent technological advancements in human pluripotent stem cell culture.
To identify key opportunities and challenges for the future therapeutic applications of hPSCs.

Main Methods:

Comprehensive literature review of technological innovations in hPSC culture.
Analysis of factors influencing hPSC culture, including media, matrices, handling, and platforms.
Synthesis of findings to assess future therapeutic potential.

Main Results:

Significant progress has been made in optimizing hPSC culture conditions.
Key technological improvements impact cell yield, quality, and differentiation potential.
Standardization of culture methods remains a challenge for widespread application.

Conclusions:

Technological advancements in hPSC culture are paving the way for clinical translation.
Addressing challenges in scalability, safety, and cost-effectiveness is critical for future therapeutic success.
Continued innovation in hPSC culture technologies will accelerate their use in regenerative medicine and drug discovery.