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Updated: Jan 3, 2026

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide
Published on: December 19, 2020
Efficiency of Penetration of Dopamine and Serotonin Peptide Derivatives through the Artificial Membranes
V P Shevchenko1, L A Andreeva1, I Yu Nagaev2
1Institute of Molecular Genetics, Russian Academy of Sciences, 123182, Moscow, Russia.
Abstract:
A mass spectrometric method has been developed for determining the content of dopamine and serotonin derivatives, which allows evaluating the efficiency of their penetration through artificial membranes depending on the structure of their peptide fragment. In this case, the diffusion of dopamine and serotonin derivatives through the membrane occurred as a result of competitive interactions. It was shown which compounds in this mixture more easily penetrate through artificial membranes. It was found that the most promising in terms of overcoming the BBB are Boc-Pro-Srt and Boc-Pro-DOPA.
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