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Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
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BODIPY-Based Fluorescent Probes for Biothiols.

Jian Zhang1, Nannan Wang1, Xin Ji2

  • 1Key Laboratory for Special Functional Materials of Ministry of Education, School of Materials Science and Engineering, Henan University, Kaifeng, 475004, P. R. China.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|November 27, 2019
PubMed
Summary

This review summarizes BODIPY-based fluorescent probes for detecting biothiols like cysteine (Cys), homocysteine (Hcy), and reduced glutathione (GSH). These probes offer advanced tools for understanding biological processes and disease states.

Keywords:
BODIPYbiothiolsfluorescent probesorganelle-targeting

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Area of Science:

  • Chemical Biology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Biothiols play crucial roles in physiological and pathological processes.
  • Fluorescent probes are essential tools for studying biothiols.
  • BODIPY fluorophores offer tunable optical properties for probe development.

Purpose of the Study:

  • To systematically review BODIPY-based fluorescent probes for biothiol detection.
  • To highlight probes for individual biothiol detection and discrimination among Cys, Hcy, and GSH.
  • To discuss organelle-targeting probes for localized biothiol analysis.

Main Methods:

  • Review of literature on BODIPY fluorophore development for biothiol sensing.
  • Analysis of probe design principles, including recognition mechanisms.
  • Discussion of biological applications and organelle targeting strategies.

Main Results:

  • BODIPY probes demonstrate significant potential for sensitive and selective biothiol detection.
  • Development of probes enabling discrimination between Cys, Hcy, and GSH.
  • Emergence of organelle-specific probes for targeted biothiol imaging.

Conclusions:

  • BODIPY-based fluorescent probes are versatile tools for biothiol research.
  • Continued development in probe design will enhance understanding of biothiol-related biological events.
  • These probes are valuable for both fundamental research and diagnostic applications.