Related Experiment Video

Updated: Jan 3, 2026

A Method of Trigonometric Modelling of Seasonal Variation Demonstrated with Multiple Sclerosis Relapse Data
10:46

A Method of Trigonometric Modelling of Seasonal Variation Demonstrated with Multiple Sclerosis Relapse Data

Published on: December 9, 2015

10.9K

Chronic kidney disease is a main confounding factor for 25-vitamin D measurement

Hanna Karla Andrade Guapyassú Machado1, Carolina Steller Wagner Martins1, Vanda Jorgetti1

  • 1Divisão de Nefrologia, Universidade de São Paulo, São Paulo, SP, Brazil.

Jornal Brasileiro De Nefrologia
|November 27, 2019
PubMed

Insights

Vitamin D testing in chronic kidney disease (CKD) patients shows significant assay variability. This impacts hypovitaminosis D diagnosis and can lead to incorrect vitamin D supplementation for CKD patients.

Area of Science:

  • Nephrology
  • Clinical Chemistry
  • Endocrinology

Background:

  • Current guidelines recommend 25-vitamin D assessment for chronic kidney disease (CKD) patients.
  • Assay variability in 25-vitamin D measurement is known, but its impact in CKD is unstudied.

Purpose of the Study:

  • To evaluate the variability between two different 25-vitamin D assays in patients with CKD.
  • To determine if CKD affects the performance and agreement of these assays.

Main Methods:

  • Consecutive 25-vitamin D measurements from 791 adult patients (122 normal renal function, 669 CKD stages 3-5/dialysis) were analyzed.
  • Measurements were taken in 2015 using Assay 1 (Diasorin LIASON) and in 2016 using Assay 2 (Beckman Coulter Unicel).

Main Results:

  • 25-vitamin D levels were lower and hypovitaminosis D prevalence higher with Assay 1 compared to Assay 2 in CKD patients.
  • Assay disagreement increased as kidney function declined.
  • These differences persisted despite similar calcium, phosphate, and parathyroid hormone levels.

Conclusions:

  • Significant variability exists between 25-vitamin D assays in CKD patients, altering hypovitaminosis D diagnosis.
  • The underlying mechanism remains unclear but may involve analytical interference.
  • This variability has clinical significance, potentially leading to erroneous vitamin D supplementation prescriptions.
Abstract

More Related Videos

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro11:30

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

Published on: June 2, 2022

2.3K
5/6 Nephrectomy Using Sharp Bipolectomy Via Midline Laparotomy in Rats05:34

5/6 Nephrectomy Using Sharp Bipolectomy Via Midline Laparotomy in Rats

Published on: April 4, 2025

1.4K

Related Concept Videos

Chronic Kidney Disease I: Introduction01:25

Chronic Kidney Disease I: Introduction

Chronic Kidney Disease (CKD) arises when the kidneys progressively lose their ability to function, ultimately leading to end-stage renal disease. At this advanced stage, the kidneys can no longer filter waste or maintain essential body functions, requiring renal replacement therapy (RRT) through dialysis or a kidney transplant for survival.Early-stage chronic kidney disease and detection challengesIn CKD's early stages, symptoms often remain absent because healthy nephrons compensate for...
516
Chronic Kidney Disease II: Clinical Manifestations01:24

Chronic Kidney Disease II: Clinical Manifestations

Chronic Kidney Disease (CKD) progressively impairs multiple body systems due to the accumulation of uremic toxins, which disrupt cellular functions across various organs.Neurologic symptomsNeurologic symptoms often arise early in CKD, as uremic toxin buildup drives changes in cognitive and motor functions. Patients frequently experience fatigue, headache, confusion, difficulty concentrating, and, in severe cases, seizures. Peripheral neuropathy commonly manifests as burning sensations in the...
495
Chronic Kidney Disease III: Interprofessional Care01:28

Chronic Kidney Disease III: Interprofessional Care

Chronic kidney disease (CKD) requires collaborative and comprehensive management. CKD progresses through stages and can lead to end-stage kidney disease (ESKD) if untreated. Interprofessional collaboration and patient education are crucial, enabling patients to manage their health and improve their quality of life.Diagnostic approach for chronic kidney diseaseThe diagnosis of CKD primarily focuses on the glomerular filtration rate (GFR), which assesses kidney function by measuring how well...
300
Drug Dosing in Renal Diseases: Dose Adjustments Based on Drug Clearance and Elimination Rate Constant01:25

Drug Dosing in Renal Diseases: Dose Adjustments Based on Drug Clearance and Elimination Rate Constant

In patients with renal disease, dosage adjustments are necessary to maintain therapeutic plasma drug concentrations and prevent toxicity or subtherapeutic exposure. Renal impairment alters drug pharmacokinetics, especially in conditions like uremia, where changes such as prolonged elimination half-life and altered apparent volume of distribution can significantly affect drug disposition. These changes require careful modification of the dosing regimen to achieve the desired clinical...
175
Drug Dosing in Renal Diseases: Measurement of Serum Creatinine Concentration and Clearance01:25

Drug Dosing in Renal Diseases: Measurement of Serum Creatinine Concentration and Clearance

In healthy individuals, serum creatinine levels remain stable due to a balance between its constant production—primarily from muscle metabolism—and renal excretion. Creatinine is freely filtered by the glomeruli, making it a valuable marker for estimating renal function. When the glomerular filtration rate (GFR) decreases, the kidneys can only eliminate less creatinine, causing serum levels to rise.Serum creatinine concentration is widely used to estimate creatinine clearance...
145
Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration01:28

Drug Dosing in Renal Diseases: Estimation of Glomerular Filtration Rate Based on Serum Creatinine Concentration

Glomerular filtration rate (GFR) can be estimated from serum creatinine using the modification of diet in renal disease (MDRD) formula or the chronic kidney disease–epidemiology collaboration (CKD–EPI) equation. Both methods are widely used in clinical practice to assess kidney function and guide treatment decisions.The MDRD equation does not require weight or height measurements and is normalized to the body surface area of 1.73 m², considered the average adult surface area.
146