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Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
Published on: October 20, 2020
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Tracking Pathogen Infections by Time-Resolved Chemical Proteomics.
Ying Zhang1,2, Der-Shyang Kao1, Bing Gu3
1Department of Biochemistry, Department of Chemistry, Center for Cancer Research, Purdue University, West Lafayette, IN, 47907, USA.
Angewandte Chemie (International Ed. in English)
|November 27, 2019
Summary
Researchers developed a new method, host and pathogen temporal interaction profiling (HAPTIP), to track pathogen entry into host cells. This technique reveals over 400 specific protein interactions during infection, enhancing our understanding of disease mechanisms.
Area of Science:
- Microbiology
- Proteomics
- Cell Biology
Background:
- Understanding host-pathogen interactions is crucial for infectious disease research.
- Current methods for studying these dynamics are often limited in temporal resolution and scope.
Purpose of the Study:
- To develop a time-resolved chemical proteomics strategy for profiling host-pathogen interactions.
- To track pathogen entry and dynamic interactions within host cells.
Main Methods:
- Introduced a novel multifunctional chemical proteomics probe for labeling bacteria.
- Utilized in vivo UV-induced crosslinking to capture interacting host-cell proteins.
- Employed label-free quantitative proteomics for interaction analysis.
Main Results:
- Successfully tracked pathogen entry into host cells using the HAPTIP strategy.
- Identified over 400 specific interacting proteins during Salmonella-containing vacuole formation.
- Generated a temporal interaction profile of host and pathogen.
Conclusions:
- The HAPTIP approach offers a high-throughput method for studying host-pathogen dynamics.
- This technique provides molecular-level insights into the infection process.
- Facilitates a deeper understanding of host-pathogen interplay during infection.
Keywords:
chemical proteomicsin vivo labelingpathogen-host interactionsphoto crosslinkingquantitative proteomicsMore Related Videos
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