Direct determination of the blood concentration of halogenated anesthetic agents by gas chromatography
Related Experiment Video
Updated: Aug 12, 2026

Biochemical Measurement of Neonatal Hypoxia
Published on: August 24, 2011
1Department of Anesthesiology and Resuscitology, Okayama University Medical School, Japan.
Abstract:
The direct determination by gas chromatography of blood levels of anesthetic agents has been difficult because of the water content of blood. In the present study, the method of Yokota et al. (1967) was modified by improving the packing materials of the column, the blood sample vaporizer and the flow-path during analysis. As a result, accurate and reproducible determination of halothane, enflurane and isoflurane dissolved in blood was achieved. With this system, blood in which halothane, enflurane and isoflurane had been dissolved could be analyzed without changing the column between samples. Moreover, each sample was prepared in less than 10 min, and more than 100 consecutive determinations could be made with excellent reproducibility. The coefficient of variation was less than 3.8%.
More Related Videos
Related Concept Videos
Gas Chromatography: Introduction
In GC, a sample is vaporized and mixed with an inert carrier gas (the mobile phase), which transports it through a column.
Gas Chromatography: Overview of Detectors
A non-destructive detector allows a sample to be analyzed without altering or consuming it, meaning the sample can be collected after detection for further analysis. Examples include thermal conductivity detectors and...
Gas Chromatography: Types of Detectors-I
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...
Gas Chromatography: Types of Detectors-II
Stages of General Anesthesia
Inhalational Anesthetics: Overview

