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Working Up Group 4 Equivocal HER2 Samples Tested by Fluorescence in Situ Hybridization in a Reference Laboratory

Katherine B Geiersbach1, Reid G Meyer1, Daniel R Sill1

  • 1Katherine B. Geiersbach, MD; Reid G. Meyer; Daniel R. Sill; Taofic Mounajjed, MD; Beiyun Chen, MD, PhD; William R. Sukov, MD; and Robert B. Jenkins, MD, PhD, Mayo Clinic School of Medicine, Rochester, MN.

Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology
|November 27, 2019
PubMed
Abstract

No abstract available in PubMed .

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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