Role of LuxR-type regulators in fish pathogenic Aeromonas hydrophila

LuxR-type transcriptional factors are essential in many bacterial physiological processes. However, there have been no reports on their roles in Aeromonas hydrophila. In this study, six stable silent strains were constructed using shRNA. Significant decreases in the expression levels of luxR₀₅ , luxR₀₈ , luxR₁₉ , luxR₁₁ , luxR₁₆₄ and luxR₁₆₅ were shown in their respective strains by qRT-PCR. The luxR₀₅ -RNAi and luxR₁₆₄ -RNAi exhibit the most significant changes in sensitivity to kanamycin and gentamicin. The luxR₀₅ -RNAi showed minimum biofilm formation and the least motility, while luxR₁₆₄ -RNAi showed minimum biofilm formation, adhesion, growth and extracellular protease activity compared to the wild-type strain. In summary, the results of this paper suggest that all six luxR genes are involved in multiple physiological processes in A. hydrophila and that the roles of luxR₀₅ and luxR₁₆₄ are highly significant. The sensitivity of luxR₀₅ -RNAi and luxR₁₆₄ -RNAi to drugs may be closely related to biofilm formation. The luxR₀₅ may play an important role in the pathogenicity of A. hydrophila by regulating the movement, adhesion and biofilm formation of bacteria, whereas luxR₁₆₄ may be involved in similar functions by regulating bacterial adhesion, extracellular enzyme activity and growth. These results help further our understanding of the drug resistance and pathogenesis of A. hydrophila.