Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

11.6K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.6K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

20.4K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
20.4K
Real Time RT-PCR02:57

Real Time RT-PCR

64.3K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
64.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Endoscopic variceal ligation versus cyanoacrylate injection for acute variceal bleeding from the cardia to the lesser curvature.

Journal of the Chinese Medical Association : JCMA·2026
Same author

Surgeon educators are infrastructure: Governing surgical training at scale.

American journal of surgery·2026
Same author

Comparing teaching methods on novice nurses' and medical residents' resuscitation teamwork knowledge and team performance: A longitudinal randomized controlled trial.

Nurse education in practice·2026
Same author

Continuous Versus Bolus Terlipressin for Acute Oesophageal Variceal Bleeding and Hepatorenal Syndrome in Cirrhosis: Meta-Analysis.

Liver international : official journal of the International Association for the Study of the Liver·2026
Same author

scDock: streamlining drug discovery targeting cell-cell communication via scRNA-seq analysis and molecular docking.

Bioinformatics (Oxford, England)·2026
Same author

Unmasking human T cell receptor germline diversity: 335 novel alleles identified in 47 Pangenome reference individuals using the gAIRR Suite.

Journal of advanced research·2026
Same journal

Therapeutic potential of crude protein extracts from two Egyptian freshwater snails Lanistes carinatus and Bellamya unicolor.

Scientific reports·2026
Same journal

Microbial contamination of donor corneas and post-keratoplasty endophthalmitis: a comparison between Japanese and U.S. eye banks using cold storage.

Scientific reports·2026
Same journal

Prevalence and contributing factors of virological non-suppression among adult patients on first-line antiretroviral therapy in tertiary hospitals in Ethiopia.

Scientific reports·2026
Same journal

An in vitro comparison of color stability between alkasite and different restorative materials in various staining solutions.

Scientific reports·2026
Same journal

Toward accessible mRNA LNP formulation: systematic evaluation of mixing strategies and key parameters.

Scientific reports·2026
Same journal

A network analysis of personality traits, mentalizing, and psychological health in Chinese college students.

Scientific reports·2026
See all related articles

Related Experiment Video

Updated: Jan 3, 2026

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms
10:41

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms

Published on: May 9, 2017

9.6K

Author Correction: Effect of de novo transcriptome assembly on transcript quantification.

Ping-Han Hsieh1, Yen-Jen Oyang1,2, Chien-Yu Chen3,4

  • 1Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei, 10617, Taiwan.

Scientific Reports
|November 28, 2019
PubMed
Summary
This summary is machine-generated.

This study has been updated. Please refer to the amendment at the top of the paper for the latest information and findings.

More Related Videos

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells
06:02

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells

Published on: October 28, 2025

352
Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

1.3K

Related Experiment Videos

Last Updated: Jan 3, 2026

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms
10:41

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms

Published on: May 9, 2017

9.6K
A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells
06:02

A Computational Pipeline for Intergenic/Intragenic Enhancer RNA Quantification in Mouse Embryonic Stem Cells

Published on: October 28, 2025

352
Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

1.3K

Area of Science:

  • Not specified in the provided abstract.

Context:

  • The original research paper has undergone an amendment.

Purpose:

  • To provide updated information and corrections to the original publication.

Summary:

  • An amendment is now available for this paper.
  • Access the amendment via the link provided at the top of the paper.

Impact:

  • Ensures readers have access to the most current and accurate information.
  • Facilitates informed understanding based on revised content.