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Updated: Jan 3, 2026
Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate
Published on: October 31, 2012
Cell therapy stimulates bone neoformation in calvaria defects in rats subjected to local irradiation
Ana Luisa Riul Sório1, Paula Katherine Vargas-Sanchez1, Roger Rodrigo Fernandes1
1Department of Basic and Oral Biology, School of Dentistry of Ribeirão Preto University of São Paulo Ribeirão Preto SP Brazil.
Background:
The purpose of the study was to analyze the effect of cell therapy on the repair process in calvaria defects in rats subjected to irradiation.
Methods:
Bone marrow mesenchymal cells were characterized for osteoblastic phenotype. Calvariae of male Wistar rats were irradiated (20 Gy) and, after 4 weeks, osteoblastic cells were placed in surgically created defects in irradiated (IRC) and control animals (CC), paired with untreated irradiated (IR) and control (C) animals. After 30 days, histological and microtomographic evaluation was performed to establish significant (P < 0.05) differences among the groups.
Results:
Higher alkaline phosphatase detection and activity, along with an increase in mineralized nodules, in the IRC, C and CC groups compared to the IR group, confirmed an osteoblastic phenotype. Histology showed impaired bone neoformation following irradiation, affecting bone marrow composition. Cell therapy in the IRC group improved bone neoformation compared to the IR group. Microtomography revealed increased bone volume, bone surface and trabecular number in IRC group compared to the IR group.
Conclusion:
Cell therapy may improve bone neoformation in defects created after irradiation.
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