Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Enzyme Kinetics

Enzyme Kinetics

Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

First total synthesis and evaluation of the α-glucosidase inhibitory activity of (±)-methyl dihydromarmesinate, an anti-inflammatory phenylpropanoid from <i>Ficus hirta</i> Vahl., and related benzofurans.

Organic & biomolecular chemistry·2026

Same author

Synthesis and evaluation of analogs of tetracyclic <i>Ganoderma</i> alkaloids as inhibitors of the enzyme α-glucosidase.

Organic & biomolecular chemistry·2025

Same author

New semisynthetic α-glucosidase inhibitor from a doubly-chemically engineered extract.

Natural products and bioprospecting·2025

Same author

1,3,4-oxadiazoles as inhibitors of the atypical member of the BET family bromodomain factor 3 from <i>Trypanosoma cruzi</i> (<i>Tc</i>BDF3).

Frontiers in microbiology·2024

Same author

Multilevel Dynamic System as Molecular Morning-After Timer.

Chemistry (Weinheim an der Bergstrasse, Germany)·2024

Same author

Thin layer chromatography assay to detect laccase inhibitors.

Food chemistry·2024

Search research articles

Related Experiment Video

Updated: Jan 3, 2026

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods

Published on: December 9, 2022

Enzymatic Bioautographic Methods.

I Ayelen Ramallo¹, Mario O Salazar¹, Ricardo L E Furlan²

¹Facultad de Ciencias Bioquímicas, Universidad Nacional de Rosario-CONICET, Rosario, Argentina.

Methods in Molecular Biology (Clifton, N.J.)

|November 28, 2019

Summary

This summary is machine-generated.

Enzymatic bioautography detects enzyme inhibitors on thin-layer chromatography plates, ideal for complex mixtures. This study details conditions for identifying xanthine oxidase and β-glucosidase inhibitors using immobilized enzymes.

Keywords:

Bioautographic method Bioguided isolation Control assay Immobilization in gel Xanthine oxidase β-Glucosidase

More Related Videos

Author Spotlight: Discovering New Biopesticides from Bioactive Soil Microbe-Derived Natural Products

Author Spotlight: Discovering New Biopesticides from Bioactive Soil Microbe-Derived Natural Products

Published on: July 26, 2024

Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans

Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans

Published on: April 28, 2023

Related Experiment Videos

Last Updated: Jan 3, 2026

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods

Published on: December 9, 2022

Author Spotlight: Discovering New Biopesticides from Bioactive Soil Microbe-Derived Natural Products

Author Spotlight: Discovering New Biopesticides from Bioactive Soil Microbe-Derived Natural Products

Published on: July 26, 2024

Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans

Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans

Published on: April 28, 2023

Area of Science:

Biochemistry
Analytical Chemistry
Enzymology

Background:

Enzymatic bioautography is a powerful technique for detecting enzyme inhibitors.
It is particularly useful for analyzing complex mixtures where inhibitors may be present.
Analyzing inhibition profiles directly from separated compounds offers significant advantages.

Purpose of the Study:

To establish conditions for detecting enzyme inhibitors using enzymatic bioautography.
To demonstrate the application of this method for identifying inhibitors of xanthine oxidase and β-glucosidase.

Main Methods:

Enzymatic bioautography was performed on thin-layer chromatography plates.
Enzymes (xanthine oxidase and β-glucosidase) were immobilized on agar gel.
Inhibition of enzyme activity by separated compounds was visualized.

Main Results:

Optimized conditions were established for the detection of enzyme inhibitors.
The method successfully identified inhibitors for both xanthine oxidase and β-glucosidase.
Enzyme inhibition profiles were generated directly from separated compounds.

Conclusions:

Enzymatic bioautography is a versatile and effective method for detecting enzyme inhibitors in complex samples.
The described conditions allow for the specific detection of xanthine oxidase and β-glucosidase inhibitors.
This technique facilitates the direct analysis of compound inhibition properties.