Related Experiment Video
Updated: Jan 3, 2026

Original Experimental Approach for Assessing Transport Fuel Stability
Published on: October 21, 2016
Oxidative properties of Moringa oleifera kernel oil from different extraction methods during storage
Masni Mat Yusoff1,2, Keshavan Niranjan2, Onyinye A Mason3
1Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Aqueous enzymatic extraction (AEE) methods preserve Moringa oleifera kernel oil
Area of Science:
- Food Science
- Lipid Chemistry
- Extraction Technology
Background:
- Moringa oleifera (MO) kernel oil is a high-oleic oil, abundant in tropical regions, offering a cost-effective source of oleic acid.
- MO oil's potential health benefits are linked to its fatty acid profile, similar to olive oil.
Purpose of the Study:
- To evaluate the impact of various extraction methods on the oxidative stability of MO kernel oil during prolonged storage.
- To determine optimal storage conditions for crude MO kernel oil before refining.
Main Methods:
- Extraction of MO kernel oil using solvent extraction (SE), aqueous enzymatic extraction (AEE), and AEE with boiling or high-pressure processing (HPP) pre-treatments.
- Storage of extracted oils for 140 days at 13°C, 25°C, and 37°C, with oxidative properties monitored.
Main Results:
- Aqueous enzymatic extraction (AEE) methods yielded MO kernel oil with superior oxidative stability and higher tocopherol retention compared to solvent extraction (SE).
- Boiling pre-treatment for AEE deactivated enzymes and preserved oil quality, while HPP accelerated hydrolysis, increasing free fatty acids.
- Tocopherol content significantly decreased in SE oil after 60 days and in AEE oils after 120 days at tested temperatures.
Conclusions:
- Extraction methods significantly influence the oxidative stability of crude MO kernel oil during storage.
- AEE methods, particularly with boiling pre-treatment, are recommended for preserving MO kernel oil quality.
- Further research into refining processes is needed for optimal application of MO kernel oil in food products.
Related Concept Videos
Radical Autoxidation
Oxidation of Alkenes: Syn Dihydroxylation with Potassium Permanganate
Oxidations of Aldehydes and Ketones to Carboxylic Acids
Aldehydes readily undergo oxidation in strong oxidizing agents such as potassium permanganate and chromic acid. The oxidation can also be carried out using mild oxidizing agents such as silver oxide. In fact, aldehydes can be easily oxidized...
Oxidation of Alkenes: Syn Dihydroxylation with Osmium Tetraoxide
Autoxidation of Ethers to Peroxides and Hydroperoxides
Oxidation of Alkenes: Anti Dihydroxylation with Peroxy Acids

