Simultaneous separation and determination of seven chelating agents using high-performance liquid chromatography...

We describe an optimization approach to determine simultaneously occurring chelating agents (glycine, malonic acid, citric acid, glycolic acid, lactic acid, DL-malic acid, and ethylenediaminetetraacetic acid) in an electroplating effluent using high-performance liquid chromatography. With chromatography signal area and overall resolution considered as responses, detection conditions were optimized via multiple functions combined with response surface methodology and Plackett-Burman design. Optimized detection conditions were as follows: 15 mmol/L ammonium phosphate buffer (pH 2.5), a 94:6 v/v ratio of ammonium phosphate buffer/acetonitrile, a column temperature of 23.3°C, and a mobile phase flow rate of 1 mL/min. The experimental values conformed to the predicted values and were repeatable (relative standard deviation < 6.4%) and linear (r²  > 0.991) over concentration ranges of 1-100 µmol/L. Moreover, the quantification limit (signal-to-noise ratio = 10) and the detection limit (signal-to-noise ratio = 3) ranged from 0.03 to 0.15 µmol/L and from 0.01 to 0.04 µmol/L, respectively. These results indicate that high-performance liquid chromatography coupled with statistical design may be a simple and rapid method for simultaneously determining multiple chelating agents in electroplating wastewater effectively.