Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Socioeconomic and lifestyle factors predict the association between sleep health and depression.

medRxiv : the preprint server for health sciences·2026
Same author

Association between traffic-related air pollution and brain morphology, assessing the olfactory pathway as a mediator.

Brain communications·2026
Same author

Upward Air Temperature Shifts and Acute Cardiovascular Events in Individuals with Atherosclerotic Cardiovascular Disease: A Time-Stratified Case-Crossover Study.

The Lancet regional health. Europe·2026
Same author

Sex-specific associations between long-term exposure to ambient air pollution and the cardiac conduction system - a longitudinal analysis of the KORA cohort.

International journal of hygiene and environmental health·2026
Same author

Determination of fluorinated pesticides in food products: Current advancements and prospective challenges.

Food chemistry·2026
Same author

Seasonal variations in hospital admissions and case-fatality of ischemic stroke: a nationwide analysis of >4.2 million cases in Germany.

Frontiers in epidemiology·2026

Related Experiment Video

Updated: Jan 3, 2026

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
12:24

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Published on: May 5, 2018

9.7K

Next-Generation Sequencing Library Preparation from FFPE Tissue Samples.

Kathrin Wolf1, Dominic O'Neil1, Stefanie Schroeer1

  • 1Qiagen, Hilden, Germany.

Current Protocols in Molecular Biology
|November 28, 2019
PubMed
Summary

This study presents a protocol for extracting and preparing DNA from formalin-fixed, paraffin-embedded (FFPE) tissues for next-generation sequencing (NGS). It addresses challenges in DNA quality and quantity for FFPE samples in molecular research.

Keywords:
formalin-fixed paraffin-embedded tissuefragmentationilluminalibrary preparationnext-generation sequencing

More Related Videos

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
07:30

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

Published on: June 8, 2020

12.6K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

12.2K

Related Experiment Videos

Last Updated: Jan 3, 2026

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
12:24

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Published on: May 5, 2018

9.7K
Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
07:30

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

Published on: June 8, 2020

12.6K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

12.2K

Area of Science:

  • Molecular biology
  • Genomics
  • Cancer research

Background:

  • Formalin-fixed, paraffin-embedded (FFPE) tissues are crucial for retrospective cancer research.
  • Utilizing FFPE samples for next-generation sequencing (NGS) presents challenges due to limited DNA quantity and quality.

Purpose of the Study:

  • To describe a protocol for DNA purification from FFPE tissues.
  • To outline DNA preparation methods for downstream NGS workflows.

Main Methods:

  • DNA purification from FFPE samples.
  • DNA fragmentation and size selection.
  • Library preparation for Illumina sequencing (MiSeq/HiSeq).

Main Results:

  • A protocol for obtaining and preparing DNA from FFPE samples for NGS was established.
  • The protocol addresses common challenges in FFPE DNA analysis.

Conclusions:

  • This protocol facilitates the use of FFPE biobanks for comprehensive molecular analysis using NGS.
  • It enables deeper insights into cancer genomics from archival samples.