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Advances and Challenges in Extremely High-Pressure Liquid Chromatography in Current and Future Analytical Scale
Ken Broeckhoven1, Gert Desmet1
1Vrije Universiteit Brussel , Department of Chemical Engineering (CHIS), Faculty of Engineering , Pleinlaan 2 , 1050 Brussels , Belgium.
Analytical Chemistry
|November 28, 2019
Summary
This study explores doubling ultrahigh-pressure liquid chromatography (UHPLC) pressures to 3000 bar for analytical scale separations. It assesses potential gains and challenges for future UHPLC instrumentation.
Area of Science:
- Analytical Chemistry
- Chromatography Science
Background:
- Current ultrahigh-pressure liquid chromatography (UHPLC) operates at 1500 bar.
- Analytical scale separations are performed using 2.1 mm columns.
Purpose of the Study:
- To evaluate the potential benefits of increasing UHPLC pressures to 3000 bar.
- To identify the primary obstacles in developing and implementing 3000 bar UHPLC systems.
Main Methods:
- Theoretical analysis of pressure-dependent separation gains.
- Review of engineering and material science challenges for high-pressure systems.
Main Results:
- Speculative gains in separation efficiency and speed at 3000 bar.
- Identification of key impediments including column integrity, pump technology, and detector compatibility.
Conclusions:
- Advancing UHPLC to 3000 bar offers significant potential for analytical separations.
- Overcoming technical hurdles is crucial for realizing the benefits of next-generation UHPLC.
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