Enhanced passivation of lead with immobilized phosphate solubilizing bacteria beads loaded with biochar/ nanoscale...

Phosphate solubilizing bacteria (PSBs) can effectively enhance the stability of lead via the formation of insoluble Pb-phosphate compounds. This research presents a bio-beads, which was implemented with the help of a self-designed porous spheres carrier, by immobilized PSBs strains Leclercia adecarboxylata (hereafter referred as L1-5). In addition, the passivation efficiency of lead via bio-beads under different conditions and its mechanism were also investigated in this study. The results indicated that phosphate solubilized by bio-beads could reach 30 mg/L in Ca₃(PO₄)₂ medium containing 1 mM Pb²⁺, and the highest removal rate of Pb²⁺ in beef peptone liquid medium could reach 93%, which is better than that of free bacteria. Furthermore, it was also concluded that the lead could be transformed into stable crystal texture, such as Pb₅(PO₄)₃Cl and Pb₅(PO₄)₃OH. Both hydrophobic and hydrophilic groups in the bio-beads could capture Pb²⁺, which indicated that electrostatic attraction and ion-exchange were also the mechanism of Pb²⁺ adsorption. All the experimental findings demonstrated that this bio-bead could be consider as an efficient way for the lead immobilization in contaminated soil in the future.