Nitrogen combined with biochar changed the feedback mechanism between soil nitrification and Cd availability in an...

Inorganic nitrogen (N) inputs increase soil nitrification, acidification and trace metal toxicity e.g. cadmium (Cd). Biochar (B) has been widely used for metal immobilization. However, little is known about how the combination of N fertilizers with biochar (N-B) changes soil Cd availability through altering nitrification process. Here, (NH₄)₂SO₄ or CO(NH₂)₂ was applied in combination with biochar to an acidic, artificially enriched Cd contaminated soil. Not as we expected, available Cd did not increase following (NH₄)₂SO₄ or CO(NH₂)₂ addition. Nitrification and acidification of Cd contaminated soils were greatly inhibited, accompanied by elimination of ammonia-oxidizing bacteria (AOB). Exchangeable H⁺ of Cd contaminated soils was significantly lower than that of uncontaminated soils, thus inhibiting Cd itself from mobilization. N-B addition nearly halved soil available Cd and significantly increased nitrification by promoting AOB recovery. However, the restored nitrification did not cause soil acidification, due to the high buffering and slow liming effects of biochar. Available Cd continuously decreased with decreasing soil acidity and exchangeable Al. This study firstly demonstrated a feedback between soil nitrification and Cd after N application, and how biochar modified the feedback. Biochar, therefore, provides a feasible strategy for eliminating potential Cd toxicity on both soil biological and chemical processes.