Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamic staff scheduling optimization algorithm for hotel management.

Scientific reports·2026
Same author

Retrospective analysis of clinical toxicology results from 2017 to 2023: exploring the characteristics and prevalence trends of detected drugs and poisons.

Journal of forensic and legal medicine·2025
Same author

Chiral analysis of ketamine enantiomers in human urine and hair: Application to authentic cases of ketamine use.

Journal of pharmaceutical and biomedical analysis·2025
Same author

Utilizing AI algorithms to model and optimize the composite of nanocellulose and hydrogels via a new technique.

International journal of biological macromolecules·2024
Same author

Econometric analysis of factors influencing electricity consumption in Spain: Implications for policy and pricing strategies.

Heliyon·2024
Same author

Discrimination of Traditional Chinese Medicine Syndromes in Type 2 Diabetic Patients Based on Metabolomics-Proteomics Profiles.

International journal of analytical chemistry·2023

Related Experiment Video

Updated: Jan 3, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

10.1K

Segmental Analysis of R/S-Methamphetamine and R/S-Amphetamine in Abusers' Head Hair.

Ting Wang1,2,3, Baohua Shen1, Hejian Wu1

  • 1Department of Forensic Toxicology, Institute of Forensic Sciences, Ministry of Justice, Shanghai Key Laboratory of Forensic Medicine, Guangfu Xi Road 1347, Shanghai 200063, China.

Journal of Analytical Toxicology
|November 29, 2019
PubMed
Summary

Segmental hair analysis reveals that increasing amphetamine (AP) to methamphetamine (MA) ratios in hair indicate greater MA dependence. This study focused on S-methamphetamine and its metabolite S-amphetamine in chronic abusers.

More Related Videos

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Published on: August 10, 2012

26.3K
A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
07:56

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

Published on: August 11, 2021

3.7K

Related Experiment Videos

Last Updated: Jan 3, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
10:17

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

10.1K
Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

Published on: August 10, 2012

26.3K
A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
07:56

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

Published on: August 11, 2021

3.7K

Area of Science:

  • Forensic Toxicology
  • Pharmacokinetics
  • Analytical Chemistry

Background:

  • Hair analysis offers a window into chronic drug use patterns.
  • Understanding the relationship between drug metabolites and dependence is crucial for treatment and legal contexts.
  • Previous studies have explored drug concentrations in hair, but segmental analysis for methamphetamine (MA) and amphetamine (AP) ratios in relation to dependence requires further investigation.

Purpose of the Study:

  • To investigate the relationship between R/S-methamphetamine (MA) and its metabolite R/S-amphetamine (AP) concentrations in hair.
  • To determine the correlation between the AP/MA ratio in segmented hair samples and MA dependence.
  • To analyze drug use patterns in chronic MA abusers through segmental hair analysis.

Main Methods:

  • Segmental hair analysis of 10 chronic MA abusers, with samples divided into 1-cm sections.
  • Quantitative measurement of MA and AP enantiomers using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS-MS).
  • Analysis of S-MA and S-AP concentrations and their ratios (S-AP/S-MA) across hair segments.

Main Results:

  • S-methamphetamine (S-MA) and S-amphetamine (S-AP) were the primary analytes detected; no R-enantiomers were found.
  • S-AP/S-MA ratios ranged from 0.03 to 0.32, suggesting predominant S-MA consumption.
  • A consistent trend of increasing S-AP/S-MA ratios from distal to proximal hair segments was observed, independent of drug concentrations.

Conclusions:

  • The increasing AP/MA ratio in hair segments correlates with the duration of MA abuse and suggests higher MA dependence.
  • No chiral conversion of MA or AP occurred within the hair matrix.
  • Segmental hair analysis confirmed continuous S-MA use and identified increased drug dosage or frequency in some users.