Lysobacter terrigena sp. nov., isolated from a Korean soil sample

Area of Science:

• Microbiology
• Bacterial Taxonomy
• Genomics

Background:

• The genus Lysobacter comprises Gram-negative bacteria known for diverse metabolic capabilities and ecological roles.
• Accurate taxonomic classification of novel bacterial isolates is crucial for understanding microbial diversity and function.

Purpose of the Study:

• To characterize a novel bacterial strain, 17J7-1^T, isolated from Jeju Island soil.
• To determine the phylogenetic and taxonomic position of strain 17J7-1^T within the genus Lysobacter.

Main Methods:

• Phenotypic characterization including morphology, growth conditions, and biochemical tests.
• Phylogenetic analysis based on 16S rRNA gene sequencing.
• Genomic analysis including DNA G+C content, DNA-DNA hybridization (DDH), and Average Nucleotide Identity (ANI) calculations.
• Fatty acid analysis and polar lipid profiling.

Main Results:

• Strain 17J7-1^T is a Gram-negative, rod-shaped, motile, yellow bacterium with optimal growth at 10–42 °C, pH 7–9, and up to 1% NaCl.
• 16S rRNA gene sequence analysis showed highest similarity to Lysobacter mobilis (97.4%).
• However, DNA-DNA relatedness (<42.3%), ANI (<77%), and dDDH (<22%) values confirmed that strain 17J7-1^T is distinct from recognized Lysobacter species, including L. mobilis and the type species L. enzymogenes.
• Major fatty acids included iso-C16:0, summed feature 9, and iso-C15:0. Phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol were the dominant polar lipids.

Conclusions:

• Strain 17J7-1^T represents a novel species within the genus Lysobacter.
• The proposed name for this new species is Lysobacter terrigena sp. nov.
• The type strain is 17J7-1^T (= KCTC 62217^T = JCM 33057^T).