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The Propagation and Quantification of Two Emerging Oncolytic Viruses: Vesicular Stomatitis (VSV) and Zika (ZIKV)

Robert E Means1, Sounak Ghosh Roy1, Samuel G Katz2

  • 1Department of Pathology, Yale School of Medicine, New Haven, CT, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 29, 2019
PubMed

Insights

Researchers are exploring viruses like vesicular stomatitis virus and Zika virus as cancer therapies. This study details methods for propagating these viruses and testing their ability to kill glioblastoma cells.

Area of Science:

  • Oncolytic virotherapy
  • Virology
  • Cancer biology

Background:

  • Genetic engineering advances enable virus-based cancer treatments.
  • Vesicular stomatitis virus (VSV) and Zika virus show promise for oncolysis.
  • Targeting cancer cells via viral lysis or immune response is a key strategy.

Purpose of the Study:

  • To establish methods for propagating and titering VSV and Zika virus.
  • To develop a cell-killing assay for evaluating modified oncolytic viruses.
  • To assess the potential of VSV and Zika virus against glioblastoma.

Main Methods:

  • Virus propagation techniques for VSV and Zika virus.
  • Viral titration assays to determine infectious units.
  • Cell-based assays to measure viral-induced glioblastoma cell death.

Main Results:

  • Protocols for reliable VSV and Zika virus propagation were established.
  • A straightforward cell-killing assay was developed.
  • Initial data suggests potential for viral-mediated glioblastoma cell killing.

Conclusions:

  • VSV and Zika virus can be effectively propagated and titered.
  • The developed assay is suitable for screening oncolytic virus efficacy.
  • Further research into modified VSV and Zika virus for glioblastoma treatment is warranted.
Keywords:
Cell killingGlioblastomaImmunotherapeuticsOncolytic virus therapyPlaque assayVesicular stomatitis virusViral titrationZika virus

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