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Radiolabeling and Imaging of Adoptively Transferred Immune Cells by Positron Emission Tomography

Amer M Najjar1

  • 1Division of Pediatrics, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. Amer.najjar@mdanderson.org.

Methods in Molecular Biology (Clifton, N.J.)
|November 29, 2019
PubMed

Positron emission tomography (PET) using 89Zr is a clinically relevant imaging modality that enables long-term monitoring of adoptively transferred immune cells. This article describes a two-step radiometal labeling procedure utilizing the bifunctional siderophore p-isothiocyanatobenzyl-desferrioxamine (DFO-Bz-NCS) that chelates 89Zr with high affinity and binds covalently to primary amines of cell-surface proteins via its isothiocyanate moiety. Cells labeled with 89Zr-DFO-Bz-NCS remain viable and retain the radiolabel, enabling repetitive PET imaging of adoptively transferred immune cells with high sensitivity and specificity for up to 2 weeks.

Keywords:
89ZrImagingImmune cellsPETPositron emission tomographyZirconiump-Isothiocyanatobenzyl-desferrioxamine

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