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An Effective Platform for Exploring Rotavirus Receptors by Bacterial Surface Display System
Danlei Liu1,2, Haoran Geng1, Zilei Zhang1,2
1Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Virologica Sinica
|November 29, 2019
Summary
This study developed a bacterial display system using Rotavirus VP8* protein to capture histo-blood group antigens (HBGAs), a key viral receptor. This method efficiently isolates viral receptors from complex mixtures, aiding in pathogen control.
Area of Science:
- Microbiology
- Virology
- Biotechnology
Background:
- Rotavirus (RV) is a significant foodborne pathogen.
- Understanding virus-receptor interactions is crucial for RV prevention.
- Purifying viral receptors like histo-blood group antigens (HBGAs) is challenging.
Purpose of the Study:
- To adapt a bacterial surface display system for Rotavirus receptor identification.
- To validate the use of RV VP8* protein in capturing HBGAs.
- To demonstrate the system's efficacy in isolating viral receptors from complex matrices.
Main Methods:
- Engineered Escherichia coli to display the Rotavirus G9P[8] VP8* protein.
- Utilized thrombin treatment for VP8* protein release while retaining antigenicity.
- Employed Western blot and ELISA to confirm protein integrity and binding.
- Tested the system's ability to capture Type A HBGAs from porcine gastric mucin and saliva.
Main Results:
- The bacterial display system successfully released functional VP8* protein.
- Type A HBGAs were specifically recognized and captured from porcine gastric mucin.
- Captured viral receptors from saliva were confirmed positive for Type A HBGAs.
- The system demonstrated efficient separation of target ligands from complex samples.
Conclusions:
- The bacterial surface display system is a feasible platform for exploring viral receptors.
- This system facilitates the isolation of viral ligands from diverse sources like cell lines and food matrices.
- The findings contribute to developing strategies for Rotavirus prevention and control.
