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Digital Planimetry for Assessing Wound Closure Kinetics in a Mouse Model
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Digital Planimetry for Assessing Wound Closure Kinetics in a Mouse Model

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Retraction

    Biofactors (Oxford, England)
    |November 29, 2019
    PubMed

    Insights

    This study investigated the K-RasG12V/T35S-ERK1/2 pathway's role in breast cancer progression. However, the article has been retracted due to image manipulation, invalidating its findings.

    Area of Science:

    • Oncology
    • Molecular Biology
    • Biochemistry

    Background:

    • The K-RasG12V/T35S-ERK1/2 pathway is implicated in cancer cell signaling.
    • Histone modifications, such as H2BS14ph, are linked to cancer progression.
    • The precise regulatory mechanisms in breast cancer remain under investigation.

    Purpose of the Study:

    • To elucidate the role of the K-RasG12V/T35S-ERK1/2 pathway in breast cancer cell advancement.
    • To investigate the involvement of Mst1 in mediating the effects of this pathway.
    • To determine the regulation of H2BS14ph by this signaling axis.

    Main Methods:

    • Western blotting to detect protein levels and phosphorylation.
    • Immunofluorescence assays for cellular localization.
    • Cell proliferation and migration assays.

    Main Results:

    • The K-RasG12V/T35S-ERK1/2 pathway was found to regulate H2BS14ph.
    • Mst1 was identified as a key mediator in this process.
    • These molecular events were associated with enhanced breast cancer cell advancement.

    Conclusions:

    • The K-RasG12V/T35S-ERK1/2 pathway, via Mst1, regulates H2BS14ph.
    • This regulatory axis promotes breast cancer cell progression.
    • NOTE: This article has been retracted due to image manipulation, rendering the conclusions invalid.

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