Related Experiment Video
Updated: Jan 3, 2026
07:54
Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy
Published on: December 15, 2011
13.4K
1H NMR-based metabolite profiling for biomarker identification.
Ruan A Silva1, Táyla C S Pereira1, Alzenir R Souza1
1Metabolomics Research Group, Departamento de Química Orgânica, Instituto de Química, Universidade Federal da Bahia, Rua Barão de Jeremoabo s/n, 40170-115 Salvador, Brazil.
Clinica Chimica Acta; International Journal of Clinical Chemistry
|November 29, 2019
Summary
Nuclear Magnetic Resonance (NMR)-based metabolomics offers a powerful approach to identify disease biomarkers in biofluids like urine and blood. This method aids in early diagnosis, prognosis, and monitoring disease progression.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Clinical Diagnostics
Background:
- The metabolome reflects physiological and environmental states, making metabolomics vital for understanding disease mechanisms.
- Advancements in analytical techniques have enabled detailed biofluid analysis for biomarker discovery.
- Nuclear Magnetic Resonance (NMR) offers rapid and comprehensive assessment of biological samples.
Purpose of the Study:
- To review recent findings in urine and blood serum/plasma metabolomics using NMR-based untargeted approaches.
- To highlight the role of metabolites as potential biomarkers for disease diagnosis, prognosis, and monitoring.
- To discuss the application of metabolomics in clinical studies for a better understanding of disease mechanisms.
Main Methods:
- Utilizing Nuclear Magnetic Resonance (NMR) spectroscopy for untargeted metabolomic profiling.
- Analyzing common biofluids including urine, plasma, and serum.
- Correlating metabolic profile alterations with human pathophysiology.
Main Results:
- NMR-based metabolomics has significantly increased in disease biomarker identification studies.
- Metabolomic profiling of biofluids can reveal potential biomarkers for early diagnosis.
- Alterations in metabolic profiles are linked to specific disease states.
Conclusions:
- NMR-based untargeted metabolomics is a crucial tool in disease research for biomarker identification.
- Metabolites in urine and blood serve as valuable biomarkers for diagnosis, therapy prediction, prognosis, and disease monitoring.
- This approach enhances understanding of disease mechanisms and facilitates personalized medicine.
Related Concept Videos
01:182D NMR: Overview of Heteronuclear Correlation Techniques
701
Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
701
01:192D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)
1.3K
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
1.3K
01:07Proton (¹H) NMR: Chemical Shift
3.1K
Organic molecules primarily contain carbon and hydrogen atoms. While all the hydrogen isotopes are NMR-active, protium or hydrogen-1 is the most abundant. It has a significant energy separation between its nuclear spin states due to its large gyromagnetic ratio. As per Boltzmann's distribution, an increase in the energy separation implies a greater excess population of nuclei available for excitation, resulting in a strong NMR absorption signal.
Absorption signals of all the protium nuclei...
Absorption signals of all the protium nuclei...
3.1K
00:58¹H NMR Signal Integration: Overview
3.2K
The intensity of a signal, which can be represented by the area under the peak, depends on the number of protons contributing to that signal. The area under each peak is shown as a vertical line called an integral, with the integral value listed under it, as seen in the proton NMR spectrum of benzyl acetate. Each integral value is divided by the smallest integral value to obtain the ratio of the number of protons producing each signal. The ratio reveals the relative number of protons and not...
3.2K
01:13¹H NMR: Complex Splitting
1.7K
A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...
1.7K
00:48¹H NMR of Labile Protons: Deuterium (²H) Substitution
1.2K
This lesson illustrates the role of deuterium substitution in simplifying the NMR spectrum of compounds comprising labile protons. One method employed is the use of deuterium. Amongst the three isotopes of hydrogen, deuterium (2H) has a nucleus composed of one proton and one neutron. When the D2O solvent is added to a pure dry ethanol solution, its labile proton is substituted with deuterium.
1.2K