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Revisiting double diffusion encoding MRS in the mouse brain at 11.7T: Which microstructural features are we sensitive
Mélissa Vincent1, Marco Palombo2, Julien Valette1
1Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), MIRCen, F-92260, Fontenay-aux-Roses, France; Neurodegenerative Diseases Laboratory, UMR9199, CEA, CNRS, Université Paris Sud, Université Paris-Saclay, F-92260, Fontenay-aux-Roses, France.
Neuroimage
|November 29, 2019
Summary
Double diffusion encoding (DDE) in NMR spectroscopy reveals brain microstructure. Incorporating branched fiber structures improves DDE signal interpretation, offering insights beyond simple fiber diameter measurements.
Area of Science:
- Neuroimaging
- Biophysical techniques
- Magnetic Resonance Imaging
Background:
- Diffusion-weighted NMR spectroscopy offers potential for cell-type specific microstructural information.
- Brain metabolites probe their cellular environment via diffusion.
- Double diffusion encoding (DDE) measures microscopic anisotropy (microA) using angular modulation.
Purpose of the Study:
- To investigate the influence of cell morphology features on DDE signal.
- To compare experimental DDE data with simulated datasets from 3D cell models.
- To refine models for interpreting DDE signals in brain microstructure.
Main Methods:
- High-accuracy DDE acquisitions in mouse brain at 11.7 T using a cryoprobe.
- State-of-the-art post-processing techniques for DDE data.
- Comparison of experimental data with simulated DDE datasets from various 3D cell models.
Main Results:
- The infinite cylinder model showed poor fit to experimental DDE data.
- Incorporating branched fiber structures into the model improved DDE signal interpretation.
- Short mixing time DDE data suggest potential sensitivity to cell body diameter.
Conclusions:
- Branched fiber structure is a critical feature for realistic DDE signal interpretation in brain microstructure.
- Current models based on infinite cylinders are insufficient for capturing DDE signal complexity.
- Further experiments are needed to confirm the sensitivity of DDE to cell body diameter.
