Spectro-electrochemical characterization and quantification of Rutin in aqueous media

Area of Science:

• Analytical Chemistry
• Electrochemistry
• Spectrophotometry

Background:

• Rutin is a flavonoid with potential health benefits.
• Understanding Rutin's chemical properties is crucial for its applications.
• Accurate quantification methods are needed for Rutin analysis.

Purpose of the Study:

• To determine the acidity constants (pK values) of Rutin in aqueous media.
• To characterize the electrochemical behavior of Rutin using a bare carbon paste electrode (CPE).
• To develop and validate analytical methods for Rutin quantification.

Main Methods:

• UV-Vis spectrophotometry was employed to measure Rutin's acidity constants and molar absorptivity.
• Electrochemical analysis of Rutin was performed using a bare CPE at neutral pH.
• Linear ranges, detection limits, and quantification limits were established for both methods.

Main Results:

• Acidity constants for Rutin were determined: pK1=4.392, pK2=7.130, pK3=8.661, pK4=12.354.
• Electrochemical oxidation and reduction of Rutin were found to be reversible, adsorption-controlled, 2-electron transfer processes.
• The bare CPE offered sensitive Rutin quantification (LOD=1.078 μM) and selectivity in the presence of ascorbic acid.

Conclusions:

• UV-Vis spectrophotometry provides detailed information on Rutin's speciation and spectral properties.
• The bare CPE is a robust and sensitive sensor for electrochemical quantification of Rutin.
• Both methods offer viable analytical approaches for Rutin determination with different sensitivities and linear ranges.