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Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells
Published on: November 18, 2016
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Cell alignment and accumulation using acoustic nozzle for bioprinting.
Yannapol Sriphutkiat1, Surasak Kasetsirikul1, Dettachai Ketpun1
1Singapore Centre for 3D Printing (SC3DP), School of Mechanical and Aerospace Engineering, Nanyang Technological University, 50 Nanyang Ave., 639798, Singapore, Singapore.
Scientific Reports
|November 30, 2019
Summary
Acoustic manipulation in bioprinting precisely patterns cells, enhancing cell-cell interactions and tissue development. This technique improves engineered tissue performance and functionality.
Area of Science:
- Biotechnology
- Tissue Engineering
- Acoustic Manipulation
Background:
- Bioprinting enables precise spatial arrangement of cells for simulating native tissues.
- Challenges include weak cell-cell interaction and slow proliferation due to uniform cell suspension in bioinks.
Purpose of the Study:
- To evaluate acoustic manipulation for cell accumulation during extrusion-based bioprinting.
- To investigate the impact of acoustic excitation on cell behavior and tissue construct development.
Main Methods:
- Extrusion-based bioprinting combined with acoustic excitation at 871 kHz.
- Utilized C2C12 cells and human umbilical vein endothelial cells (HUVECs).
- Assessed cell distribution, viability, morphology, and differentiation post-printing.
Main Results:
- Acoustic excitation significantly reduced cell distribution width (2.2-fold FWHM reduction).
- Enhanced C2C12 cell alignment and elongation, with decreased circularity.
- Promoted capillary-like structure formation and neovascularization in HUVECs.
Conclusions:
- Acoustic technology effectively accumulates and patterns cells in bioprinted constructs with high biocompatibility.
- Improved cell interaction and differentiation enhance the functionality of engineered tissues.
- Acoustic bioprinting offers a promising approach for advanced tissue engineering applications.
