Related Experiment Video

Updated: Jan 2, 2026

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
08:22

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

8.5K

Distinct Circulating Expression Profiles of Long Noncoding RNAs in Heart Failure Patients With Ischemic and

Fang Lin1,2,3, Xin Gong4, Ping Yu4

  • 1Research Center for Translational Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.

Frontiers in Genetics
|November 30, 2019
PubMed

Insights

This study reveals distinct long noncoding RNA (lncRNA) and messenger RNA (mRNA) profiles in plasma for ischemic cardiomyopathy (ICM) and dilated cardiomyopathy (DCM) patients. These findings may aid in diagnosing heart failure etiology and improving prognosis.

Area of Science:

  • Cardiovascular Biology
  • Molecular Genetics
  • Biomarker Discovery

Background:

  • Ischemic cardiomyopathy (ICM) and dilated cardiomyopathy (DCM) are leading causes of heart failure (HF).
  • Distinct prognoses and treatment responses necessitate accurate etiological diagnosis.
  • Plasma-based biomarkers for differentiating ICM and DCM are currently lacking.

Purpose of the Study:

  • To investigate differential long noncoding RNA (lncRNA) and messenger RNA (mRNA) expression profiles in plasma from DCM and ICM patients.
  • To identify specific lncRNA signatures that distinguish between DCM and ICM.
  • To explore the functional implications of these differentially expressed RNAs in heart failure pathogenesis.

Main Methods:

  • Plasma samples from DCM and ICM patients were analyzed using microarray technology.
  • Differential expression analysis was performed for lncRNAs and mRNAs.
  • Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was conducted on coexpressed genes.
  • Quantitative real-time PCR (qRT-PCR) was used to validate key lncRNA findings.

Main Results:

  • Microarray analysis identified 3,222 differentially expressed lncRNAs and 1,911 differentially expressed mRNAs between DCM and ICM groups.
  • Upregulated functional terms included positive regulation of I-kappaB kinase/nuclear factor-kappaB signaling.
  • Differentially expressed lncRNA-coexpressed mRNAs were enriched in pathways such as natural killer cell mediated cytotoxicity and ras signaling.

Conclusions:

  • This study presents the first comprehensive analysis of plasma lncRNA and mRNA profiles in DCM and ICM patients.
  • Specific expression patterns of lncRNAs and mRNAs can differentiate between DCM and ICM.
  • These findings offer potential biomarkers for etiological diagnosis and prognosis evaluation in heart failure.

Keywords:
dilated cardiomyopathyexpression profileischemic cardiomyopathylong noncoding RNAmessenger RNAmicroarray

More Related Videos

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease04:41

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Published on: July 3, 2018

8.6K
Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion07:54

Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Published on: August 29, 2018

8.9K

Related Concept Videos

Cardiomyopathy II: Dilated Cardiomyopathy01:30

Cardiomyopathy II: Dilated Cardiomyopathy

Dilated cardiomyopathy, or DCM, is a progressive myocardial disorder characterized by ventricular chamber dilation and contractile dysfunction.EtiologyVarious factors can cause DCM, including hypertension and heavy alcohol intake, which contribute to the weakening and enlargement of the heart muscle. Viral infections, such as Coxsackievirus B, adenoviruses, and influenza, can lead to DCM by causing inflammation and damage to heart tissue. Certain chemotherapeutic agents, including daunorubicin,...
405
Pathophysiology of Heart Failure01:17

Pathophysiology of Heart Failure

Heart failure (HF) is a progressive syndrome involving ventricles that leads to inadequate cardiac output. It can be classified based on location and output or ejection fraction. Ejection fraction (EF) is an essential measurement in the diagnosis and surveillance of HF. Reduced EF corresponds to systolic heart failure (HFrEF). However, HF with preserved ejection fraction (HFpEF) is becoming increasingly prevalent. Also known as diastolic HF, this form of HF is related to aging. The...
2.6K
Cardiomyopathy IV: Restrictive Cardiomyopathy01:29

Cardiomyopathy IV: Restrictive Cardiomyopathy

Restrictive cardiomyopathy (RCM) is a rare heart muscle disease characterized by impaired ventricular filling due to stiffened ventricular walls, leading to significant diastolic dysfunction.EtiologyRestrictive cardiomyopathy can arise from both inherited and acquired diseases, many of which are systemic. It is categorized into four main types: infiltrative, storage, non-infiltrative, and endomyocardial diseases.Infiltrative diseases, such as amyloidosis, lead to RCM by depositing amyloid...
402
Heart Failure II: Pathophysiology01:29

Heart Failure II: Pathophysiology

Systolic Heart Failure and Compensatory MechanismsSystolic heart failure (also termed HFrEF, Heart Failure with Reduced Ejection Fraction) is the most prevalent type of heart filure. It results in a decreased volume of blood being pumped from the ventricle. The aortic arch and carotid sinuses have baroreceptors that detect reduced blood pressure, triggering the sympathetic nervous system (SNS) to release epinephrine and norepinephrine. Initially, this response aims to boost heart rate and...
624
Cardiomyopathy III: Hypertrophic Cardiomyopathy01:29

Cardiomyopathy III: Hypertrophic Cardiomyopathy

Hypertrophic cardiomyopathy, or HCM, is an autosomal dominant genetic disorder characterized by asymmetric left ventricular hypertrophy without ventricular dilation. It is more common in men and is typically diagnosed in young, athletic adults.EtiologyHCM is primarily genetic and is caused by mutations in genes encoding sarcomeric proteins. Researchers have identified over 1400 mutations across at least 11 different genes. Among these, the most frequently occurring mutations are found in the...
325
Cardiomyopathy I: Introduction and Classification01:25

Cardiomyopathy I: Introduction and Classification

Cardiomyopathy, or CMP, is a group of diseases affecting the myocardial structure, impairing its ability to pump blood effectively. This condition can lead to arrhythmias, heart failure, or sudden cardiac death.Cardiomyopathies are classified into primary and secondary categories:Primary Cardiomyopathy refers to conditions involving only the heart muscle that are often idiopathic (of unknown cause) or genetic. They primarily affect the myocardium without the involvement of other systemic...
442