Does glucose affect the de-esterification of methyl ferulate by Lactobacillus buchneri?
Kamyar Mogodiniyai Kasmaei1, Dietmar Schlosser2, Heike Sträuber2
1Department of Animal Nutrition and management, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Microbiologyopen
|November 30, 2019
Summary
This study investigated if glucose represses feruloyl esterase (FAE) activity in Lactobacillus buchneri LN 4017 during silage fermentation. Results show glucose does not inhibit FAE activity, suggesting other factors influence fiber utilization in silage.
Area of Science:
- Agricultural Science
- Microbiology
- Biochemistry
Background:
- Silage production involves anaerobic storage of forage crops.
- Lactobacillus buchneri LN 4017 produces feruloyl esterase (FAE) to break down plant fibers.
- Previous attempts to improve silage fiber utilization with this strain yielded inconsistent results.
Purpose of the Study:
- To test the hypothesis that glucose represses FAE activity in Lactobacillus buchneri LN 4017.
- To understand the inconsistent effects of this bacterium on silage fiber digestibility.
Main Methods:
- Assessed the effect of glucose on the de-esterification of methyl ferulate (MF) by Lactobacillus buchneri LN 4017.
- Utilized three different glucose:MF ratios (0:1, 1:1, and 13:1) to evaluate FAE activity.
Main Results:
- Lactobacillus buchneri LN 4017 continued to hydrolyze methyl ferulate (MF) even at high glucose concentrations.
- Increasing glucose to MF ratios did not repress the de-esterification activity of the bacteria.
Conclusions:
- Glucose does not catabolically repress feruloyl esterase (FAE) activity in Lactobacillus buchneri LN 4017.
- The inconsistent effects of this strain on silage fiber digestibility are not due to glucose repression of FAE activity.
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