Adaptation of euhalophyte Suaeda salsa to nitrogen starvation under salinity

Suaeda salsa L. (S. salsa) is an annual euhalophyte with high salt tolerance. The NO₃^- content in soils where S. salsa populations occur are very low, especially in intertidal habitat. However, it remains unclear how S. salsa populations adapt to low nitrogen environments. Plants of two S. salsa populations were pre-cultured with nitrate nitrogen (1 mM of NO₃^--N) for 30 days. Then, the seedlings were cultured with 1 mM of NO₃^--N and N-free solution (N starvation) at 200 mM of NaCl for an additional 14 days. The expression of two genes in S. salsa, nitrate transporter 1.7 (SsNRT1.7) and nitrate transporter 2.5 (SsNRT2.5) in old and mature leaves, was markedly upregulated during N starvation in the intertidal population, when compared to the inland population, but this was not the case in young leaves. After N starvation, the decrease in NO₃^- and chlorophyll content, net photosynthetic rate in young leaves, and shoot dry weight in the intertidal population were lower than those in the inland population. In conclusion, SsNRT1.7 and SsNRT2.5 may play a role in NO₃^- remobilization, especially in the intertidal population, during N starvation. This trait may benefit the intertidal population for adapting to low nitrogen environments.