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Updated: Jan 2, 2026
Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
Published on: November 1, 2012
Nickel complexes of aroylhydrazone ligand: synthesis, crystal structure and DNA binding properties
Ping Yang1, Li-Lei Zhang2, Zi-Zhou Wang3
1Guangdong Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China; State Key Laboratory of Applied Microbiology Southern China, China; Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,China; Guangdong Open Laboratory of Applied Microbiology, China.
Abstract:
In this work, three aroylhydrazone ligands ((E)-2-hydroxy-N'-(1-(pyrazin-2-yl)ethylidene)benzohydrazide, HL1; (E)-3-hydroxy-N'-(1-(pyrazin-2-yl)ethylidene)benzohydrazide, HL2; and (E)-4-hydroxy-N'-(1-(pyrazin-2-yl)ethylidene)benzohydrazide, HL3) and their complexes with nickel (Ni(L1)2, NiL1; Ni(L2)2∙2DMF, NiL2; Ni(L3)2∙2DMF, NiL3) were prepared. The single crystal X-ray structures analysis of three compounds showed that they were neutral. The ligand adopts tridentate chelating mode. The nickel ion is six-coordinate with two O atoms and four N atoms from two ligands, and forms an octahedral arrangement. The investigation of DNA binding ability by ultraviolet and fluorescence titrations showed that NiL2 and NiL3 exhibit moderate binding affinity toward calf Thymus DNA. Spectroscopy, molecular docking, and molecular dynamics simulation indicated that NiL2 and NiL3 bind at the minor groove of DNA through intercalation.
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