Neddylation regulates excitatory synaptic transmission and plasticity
Marisa M Brockmann1,2,3, Michael Döngi1, Ulf Einsfelder1
1Institut für Physiologie II, Universität Bonn, Bonn, Germany.
Scientific Reports
|December 1, 2019
Summary
Neddylation, a protein modification, is crucial for synaptic transmission and plasticity. Inhibiting neddylation acutely affects neurotransmission and synaptic plasticity by impacting presynaptic release and postsynaptic receptor stabilization.
Area of Science:
- Neuroscience
- Molecular Biology
- Cell Biology
Background:
- Post-translational modifications (PTMs) regulate synaptic function by altering protein properties.
- Neddylation, a PTM involving Nedd8 conjugation, impacts neuronal maturation and spine stability.
Purpose of the Study:
- To investigate the role of neddylation in excitatory synaptic transmission and plasticity.
- To identify synaptic targets regulated by the neddylation pathway.
Main Methods:
- Acute inhibition of the neddylation pathway in neurons.
- Electrophysiological recordings to assess synaptic transmission and plasticity.
- Analysis of presynaptic release probability and postsynaptic receptor stabilization.
Main Results:
- Acute neddylation inhibition impaired synaptic neurotransmission prior to morphological changes.
- Neddylation affects both presynaptic release probability and postsynaptic receptor stabilization.
- Blocking neddylation abolished long-term potentiation and long-term depression.
Conclusions:
- Neddylation is essential for regulating excitatory synaptic transmission and plasticity.
- Identifying synaptic neddylation targets is key to understanding synaptic regulation.
Related Concept Videos
01:35Long-term Potentiation
58.0K
Long-term potentiation, or LTP, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTP is the process of synaptic strengthening that occurs over time between pre- and postsynaptic neuronal connections. The synaptic strengthening of LTP works in opposition to the synaptic weakening of long-term depression (LTD) and together are the main mechanisms that underlie learning and memory.
58.0K
01:25Long-term Potentiation
3.3K
Long-term potentiation, or LTP, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTP is the process of synaptic strengthening that occurs over time between pre and postsynaptic neuronal connections. The synaptic strengthening of LTP works in opposition to the synaptic weakening of long-term depression (LTD) and together are the main mechanisms that underlie learning and memory.
Hebbian LTP
LTP can occur when...
Hebbian LTP
LTP can occur when...
3.3K
01:01Neuroplasticity
1.4K
Neuroplasticity reflects the brain's remarkable capacity to adapt and evolve, responding dynamically to learning, experiences, or injury by reorganizing its neural circuitry. This reorganization involves creating new neural connections and refining old ones through a series of biological processes that contribute to the brain's lifelong development and adaptability.
1.4K
01:29Excitatory and Inhibitory Effects of Neurotransmitters
12.4K
When an action potential reaches the presynaptic axon terminal, it releases neurotransmitters from the neuron into the synaptic cleft at a chemical synapse. The released neurotransmitter can be excitatory or inhibitory. The critical criteria commonly used to determine whether a molecule is a neurotransmitter at a chemical synapse are the molecule's presence in the presynaptic neuron. Second, its release is in response to strong presynaptic depolarization. And lastly, the presence of...
12.4K
01:03Long-term Depression
3.0K
Long-term depression, or LTD, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTD is the process of synaptic weakening that occurs over time between pre and postsynaptic neuronal connections. The synaptic weakening of LTD works in opposition to synaptic strengthening by long-term potentiation (LTP) and together are the main mechanisms that underlie learning and memory.
Calcium Ion Concentration Mechanism
If over...
Calcium Ion Concentration Mechanism
If over...
3.0K
01:05Long-term Depression
32.9K
Long-term depression, or LTD, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTD is the process of synaptic weakening that occurs over time between pre and postsynaptic neuronal connections. The synaptic weakening of LTD works in opposition to synaptic strengthening by long-term potentiation (LTP) and together are the main mechanisms that underlie learning and memory.
32.9K