Probiotics ingestion prevents HDAC11-induced DEC205+ dendritic cell dysfunction in night shift nurses

Area of Science:

• Immunology
• Chronobiology
• Microbiology

Background:

• Shift work disrupts circadian rhythms and negatively impacts the immune system.
• The precise mechanisms of immune dysfunction due to shift work require further investigation.
• Probiotics are known to modulate immune functions.

Purpose of the Study:

• To investigate the protective role of probiotic (C. Butyricum) yogurt against day-night shift-induced dysfunction of DEC205+ dendritic cells (decDCs) in nurses.
• To explore the molecular mechanisms underlying decDC impairment in shift-working nurses.

Main Methods:

• Blood samples were collected from nurses working day-night shifts, with some consuming probiotic yogurt during night shifts.
• Immunological assays were used to evaluate the function of decDCs.
• Levels of HDAC11 and Bmal1 were assessed in decDCs.

Main Results:

• Night shifts impaired the immune tolerogenic functions and IL-10 expression in decDCs.
• HDAC11 was upregulated in decDCs after night shifts and correlated negatively with immune function.
• Probiotic yogurt consumption suppressed Bmal1 and HDAC11 levels and restored decDC immune regulatory functions.

Conclusions:

• Day-night shift work negatively affects the immune tolerogenic functions of decDCs.
• Probiotic yogurt ingestion during night shifts can prevent shift work-induced decDC dysfunction.
• This suggests a potential dietary intervention to mitigate immune disruption from shift work.