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Hsa_circ_0000285 functions as a competitive endogenous RNA to promote osteosarcoma progression by sponging

Zhicai Zhang1, Feifei Pu1, Baichuan Wang1

  • 1Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.

Gene Therapy
|December 1, 2019
PubMed

Insights

Circular RNA (circ-0000285) promotes osteosarcoma progression by enhancing cell proliferation and migration. This effect is mediated through the circ-0000285/miRNA-599/TGFB2 axis, offering new insights into osteosarcoma pathogenesis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) play a role in disease pathogenesis.
  • Osteosarcoma is a bone cancer where circRNAs are implicated.
  • hsa_circ_0000285 (circ-0000285) is upregulated in osteosarcoma and affects cell behavior.

Purpose of the Study:

  • To investigate the role of circ-0000285 in osteosarcoma cell proliferation and migration.
  • To elucidate the molecular mechanism involving circ-0000285, miRNA-599, and TGFB2 in osteosarcoma progression.

Main Methods:

  • Gene expression analysis using qRT-PCR to compare circ-0000285 levels in osteosarcoma and normal cells.
  • Dual-luciferase reporter gene assay and RIP assay to confirm binding interactions between circ-0000285, miRNA-599, and TGFB2.
  • EdU assay and transwell migration assay to evaluate the functional impact of circ-0000285 and miRNA-599 on cell proliferation and migration.

Main Results:

  • circ-0000285 was found to be highly expressed in osteosarcoma cell lines compared to normal osteoblasts.
  • circ-0000285 and TGFB2 were confirmed to directly bind to miRNA-599.
  • Overexpression of circ-0000285 increased osteosarcoma cell proliferation and migration, effects reversed by miRNA-599 overexpression.

Conclusions:

  • The circ-0000285/miRNA-599/TGFB2 axis is a critical regulator of osteosarcoma cell proliferation and migration.
  • This axis provides a novel perspective for understanding osteosarcoma pathogenesis.
  • circ-0000285 may serve as a potential therapeutic target for osteosarcoma.

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