Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Neurogenesis and Regeneration of Nervous Tissue01:15

Neurogenesis and Regeneration of Nervous Tissue

1.5K
In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...
1.5K
Whole Body Regeneration01:33

Whole Body Regeneration

4.0K
Regeneration is the process of restoring injured or lost tissues, organs, or body parts. While simpler organisms generally show greater ability to regenerate their whole body, few complex animals show similarly exceptional regeneration. For example, planarian flatworms have a unique regenerative potential making them a popular study organism among biologists to understand the mechanisms of whole body regeneration. Other organisms, such as hydra, also show extreme regeneration potential;...
4.0K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

2.1K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.1K
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

2.2K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
2.2K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.5K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A 3D amphioxus brain atlas illuminates the blueprint of the ancestral chordate brain and highlights unique vertebrate innovations.

Science advances·2026
Same author

Phenotypic Response Surfaces-Guided Optimization (PRS-OPT) of Propolis-Metformin-Regorafenib Combination Therapy for MASLD-Associated Hepatocellular Carcinoma.

Oncology research·2026
Same author

Zebrafish knock-in lines enabling live visualization of extracellular matrix dynamics during development and regeneration.

Development (Cambridge, England)·2026
Same author

In vivo single-cell RNA metabolic labeling resolves early transcriptional responders in the regenerating zebrafish heart.

Nature communications·2026
Same author

Enhancer-directed gene delivery for digit regeneration based on conserved epidermal factors.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Insights into cephalochordate genome and gene evolution from the early-diverging amphioxus <i>Asymmetron lucayanum</i>.

Proceedings of the National Academy of Sciences of the United States of America·2026

Related Experiment Video

Updated: Jan 2, 2026

De Novo Generation of Somatic Stem Cells by YAP/TAZ
13:05

De Novo Generation of Somatic Stem Cells by YAP/TAZ

Published on: May 7, 2018

9.5K

Genetic Reprogramming of Positional Memory in a Regenerating Appendage.

Ying-Ting Wang1, Tzu-Lun Tseng1, Yu-Chia Kuo1

  • 1Institute of Cellular and Organismic Biology, Academia Sinica, Taipei 11529, Taiwan.

Current Biology : CB
|December 2, 2019
PubMed
Summary

Zebrafish regeneration size is determined by proliferating cells during a critical window. Disrupting this process leads to miniaturized fins, showing regeneration is adaptable and evolutionarily conserved.

Keywords:
forward geneticspola2positional informationregenerationzebrafish

More Related Videos

Author Spotlight: Mapping Cellular Connectivity in the Zebrafish Nervous System During Development and Regeneration
06:08

Author Spotlight: Mapping Cellular Connectivity in the Zebrafish Nervous System During Development and Regeneration

Published on: July 5, 2024

1.5K
Chemical Amputation and Regeneration of the Pharynx in the Planarian Schmidtea mediterranea
06:14

Chemical Amputation and Regeneration of the Pharynx in the Planarian Schmidtea mediterranea

Published on: March 26, 2018

11.4K

Related Experiment Videos

Last Updated: Jan 2, 2026

De Novo Generation of Somatic Stem Cells by YAP/TAZ
13:05

De Novo Generation of Somatic Stem Cells by YAP/TAZ

Published on: May 7, 2018

9.5K
Author Spotlight: Mapping Cellular Connectivity in the Zebrafish Nervous System During Development and Regeneration
06:08

Author Spotlight: Mapping Cellular Connectivity in the Zebrafish Nervous System During Development and Regeneration

Published on: July 5, 2024

1.5K
Chemical Amputation and Regeneration of the Pharynx in the Planarian Schmidtea mediterranea
06:14

Chemical Amputation and Regeneration of the Pharynx in the Planarian Schmidtea mediterranea

Published on: March 26, 2018

11.4K

Area of Science:

  • Regenerative Biology
  • Developmental Biology
  • Evolutionary Biology

Background:

  • Vertebrates like salamanders and zebrafish can regenerate complex tissues.
  • The mechanism by which stump cells recall positional information for regeneration has been a long-standing question.

Purpose of the Study:

  • To investigate how positional information, specifically size, is encoded during appendage regeneration.
  • To identify genetic and cellular mechanisms controlling regenerative patterning.

Main Methods:

  • Chemical mutagenesis screen in zebrafish to identify genes affecting fin regeneration.
  • Temperature-shift assays using a temperature-sensitive mutant of DNA polymerase alpha subunit 2 (pola2).
  • Progenitor cell proliferation disruption in zebrafish, amphioxus, and annelids.

Main Results:

  • A critical 48-hour window in zebrafish fin regeneration was identified where size information is encoded.
  • Mutants in pola2, affecting DNA replication, regenerated miniaturized fins when manipulated during this window.
  • Disrupting progenitor cell proliferation transiently altered regenerative size in various species, with memory of the new size observed.

Conclusions:

  • Positional information during regeneration is not fixed but malleable, influenced by progenitor cell proliferation.
  • This adaptable mechanism for controlling regenerative size appears to be evolutionarily conserved across diverse phyla.