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Related Experiment Video

Updated: Jan 2, 2026

Author Spotlight: Dendritic Cells Maturation Using Sialidases-Based Enzymatic Treatment of the Cell Surface
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Intracellular Galectin-9 Controls Dendritic Cell Function by Maintaining Plasma Membrane Rigidity.

Laia Querol Cano1, Oya Tagit1, Yusuf Dolen1

  • 1Department of Tumour Immunology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Geert Grooteplein 26-28, Nijmegen 6525 GA, The Netherlands.

Iscience
|December 2, 2019
PubMed
Summary
This summary is machine-generated.

Intracellular Galectin-9 maintains dendritic cell (DC) membrane structure and function. This protein is essential for pathogen uptake via C-type lectin receptors, highlighting its critical role in DC immunity.

Keywords:
Biological SciencesCell BiologyMolecular Biology

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Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Galectins are known extracellular proteins involved in cell surface organization.
  • The intracellular functions of Galectins, particularly Galectin-9, remain largely unexplored.
  • Dendritic cells (DCs) play a crucial role in initiating immune responses.

Purpose of the Study:

  • To investigate the role of intracellular Galectin-9 in dendritic cell (DC) surface organization and function.
  • To elucidate the molecular mechanisms underlying Galectin-9's effects on DC plasma membrane structure and phagocytosis.

Main Methods:

  • Functional assays
  • Super-resolution microscopy
  • Atomic force microscopy to assess membrane stiffness
  • Galectin-9 knockdown studies
  • Rac1 activity assays
  • Phagocytosis assays using C-type lectin receptors
  • Analysis of Galectin-9-null murine DCs

Main Results:

  • Intracellular Galectin-9 is essential for maintaining plasma membrane integrity and structure in DCs.
  • Galectin-9 modulates Rac1 activity, directly controlling cortical membrane structure and actin cytoskeleton organization.
  • DCs lacking Galectin-9 exhibit impaired phagocytic capacity, particularly for C-type lectin receptor-mediated pathogen uptake.

Conclusions:

  • Intracellular Galectin-9 plays a novel and critical role in regulating DC plasma membrane organization and function.
  • Galectin-9 is indispensable for efficient phagocytosis mediated by C-type lectin receptors in DCs.
  • The identified intracellular functions of Galectin-9 may represent an evolutionarily conserved mechanism in immune cells.