Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Pulmonary Tuberculosis IV

Pulmonary Tuberculosis IV

Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...

Serum Laboratory Studies, Stool Test, Breath Test

Serum Laboratory Studies, Stool Test, Breath Test

Gastrointestinal (GI) diagnostic studies are pivotal in confirming, ruling out, diagnosing, or staging various diseases, including cancers. Following diagnosis, allocating time for discussions with the patient and providing informational resources is crucial. Diagnostic assessments of the GI tract often occur in outpatient settings like endoscopy suites or GI labs. Preparation for these tests may include dietary restrictions, fasting, liquid bowel preparations, laxatives, enemas, and the...

Pulmonary Tuberculosis I

Pulmonary Tuberculosis I

Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Integrated assessment of thiram toxicity in pangasius fish: Erythrocytic abnormalities, gill and intestinal defense mechanisms, and genotoxicity.

PloS one·2026

Same author

Correlation of the monocyte to high-density lipoprotein cholesterol ratio and neutrophil to high-density lipoprotein cholesterol ratio with the severity of acute ischemic stroke.

eNeurologicalSci·2026

Same author

Prospective Observational Outcomes after Anterior Cervical Discectomy and Fusion.

Journal of the College of Physicians and Surgeons--Pakistan : JCPSP·2026

Same author

Small polaron-mediated zero differential transconductance of 2D semiconductor/CoFe<sub>2</sub>O<sub>4</sub> heterojunctions for plateau transistor applications.

Nature communications·2026

Same author

Blue-LED Photocatalytic Synthesis of Large-Area Benzobisazole-Based 2D Zwitterionic Polymer Membranes for Durable Proton Exchange.

ACS applied materials & interfaces·2026

Same author

Chitosan-quercetin complex containing nanospheres for targeted delivery of methotrexate against MCF-7 breast cancer cells.

International journal of biological macromolecules·2026

Search research articles

Related Experiment Video

Updated: Jan 2, 2026

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test

Published on: April 9, 2012

GeneXpert in stool: Diagnostic yield in Intestinal Tuberculosis.

Abu Talib¹, Shaheen Bhatty¹, Khalid Mehmood¹

¹Dow Medical College, Dow University of Health Sciences., Baba-e-Urdu Road, Karachi 74200, Pakistan.

Journal of Clinical Tuberculosis and Other Mycobacterial Diseases

|December 3, 2019

Summary

Diagnosing intestinal tuberculosis using stool GeneXpert assay is a rapid alternative. This method shows good accuracy, aiding early detection and management of intestinal tuberculosis.

Keywords:

GeneXpert MTB/RIF assay Ileocaecal tuberculosis Mycobacterium tuberculosis Nucleic Acid Amplification Techniques

More Related Videos

Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection

Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection

Published on: February 16, 2020

Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection

Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection

Published on: June 8, 2020

Related Experiment Videos

Last Updated: Jan 2, 2026

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test

Published on: April 9, 2012

Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection

Synthesis, Characterization, and Application of Superparamagnetic Iron Oxide Nanoprobes for Extrapulmonary Tuberculosis Detection

Published on: February 16, 2020

Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection

Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection

Published on: June 8, 2020

Area of Science:

Infectious Diseases
Microbiology
Gastroenterology

Background:

Diagnosing intestinal tuberculosis is difficult due to low yields from CT scans and colonoscopies.
GeneXpert MTB/RIF (Xpert) assays show promise for diagnosing tuberculosis in various body secretions.

Purpose of the Study:

To evaluate the diagnostic yield and accuracy of the GeneXpert assay when performed on stool samples from patients with suspected intestinal tuberculosis.

Main Methods:

One hundred patients underwent standard diagnostic tests including colonoscopy and biopsies.
Fresh stool samples were collected and tested using the GeneXpert assay for Mycobacterium tuberculosis and Rifampicin resistance.

Main Results:

GeneXpert assay on stool was positive in 20% of suspected intestinal tuberculosis cases.
Compared to histopathology, stool GeneXpert showed a sensitivity of 39.1% and specificity of 85.7%.

Conclusions:

Stool GeneXpert assay provides a rapid alternative for detecting intestinal tuberculosis with acceptable accuracy.
While not replacing AFB culture or histopathology, it can contribute to earlier diagnosis and treatment initiation.