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Published on: April 9, 2012
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GeneXpert in stool: Diagnostic yield in Intestinal Tuberculosis
Abu Talib1, Shaheen Bhatty1, Khalid Mehmood1
1Dow Medical College, Dow University of Health Sciences., Baba-e-Urdu Road, Karachi 74200, Pakistan.
Journal of Clinical Tuberculosis and Other Mycobacterial Diseases
|December 3, 2019
Summary
Diagnosing intestinal tuberculosis using stool GeneXpert assay is a rapid alternative. This method shows good accuracy, aiding early detection and management of intestinal tuberculosis.
Area of Science:
- Infectious Diseases
- Microbiology
- Gastroenterology
Background:
- Diagnosing intestinal tuberculosis is difficult due to low yields from CT scans and colonoscopies.
- GeneXpert MTB/RIF (Xpert) assays show promise for diagnosing tuberculosis in various body secretions.
Purpose of the Study:
- To evaluate the diagnostic yield and accuracy of the GeneXpert assay when performed on stool samples from patients with suspected intestinal tuberculosis.
Main Methods:
- One hundred patients underwent standard diagnostic tests including colonoscopy and biopsies.
- Fresh stool samples were collected and tested using the GeneXpert assay for Mycobacterium tuberculosis and Rifampicin resistance.
Main Results:
- GeneXpert assay on stool was positive in 20% of suspected intestinal tuberculosis cases.
- Compared to histopathology, stool GeneXpert showed a sensitivity of 39.1% and specificity of 85.7%.
Conclusions:
- Stool GeneXpert assay provides a rapid alternative for detecting intestinal tuberculosis with acceptable accuracy.
- While not replacing AFB culture or histopathology, it can contribute to earlier diagnosis and treatment initiation.
Keywords:
GeneXpert MTB/RIF assayIleocaecal tuberculosisMycobacterium tuberculosisNucleic Acid Amplification TechniquesMore Related Videos
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