Reaction-based indicator displacement assay (RIA) for the development of a triggered release system capable of biofilm inhibition
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Updated: Jul 11, 2026

Microtiter Dish Biofilm Formation Assay
Published on: January 30, 2011
Bethany L Patenall1, George T Williams, Lauren Gwynne
1Department of Chemistry, University of Bath, Bath, BA2 7AY, UK. t.d.james@bath.ac.uk A.T.A.Jenkins@bath.ac.uk.
Abstract:
Here, a reaction-based indicator displacement hydrogel assay (RIA) was developed for the detection of hydrogen peroxide (H2O2) via the oxidative release of the optical reporter Alizarin Red S (ARS). In the presence of H2O2, the RIA system displayed potent biofilm inhibition for Methicillin-resistant Staphylococcus aureus (MRSA), as shown through an in vitro assay quantifying antimicrobial efficacy. This work demonstrated the potential of H2O2-responsive hydrogels containing a covalently bound diol-based drug for controlled drug release.
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