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The potential influence of long non-coding RNA PRKG1-AS1 on oral squamous cell carcinoma: A comprehensive study based

Ting Wu1, Shi-Yang Zhang1, Wen-Jie Dong1

  • 1Department of Dental, Second Affiliated Hospital of Mudanjiang Medical University, Mudanjiang, China.

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
|December 3, 2019
PubMed
Abstract

Insights

Long non-coding RNA PRKG1-AS1 promotes oral squamous cell carcinoma (OSCC) progression by enhancing cell growth, invasion, and migration. High PRKG1-AS1 expression correlates with poor patient outcomes, suggesting its role in OSCC development.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Oral squamous cell carcinoma (OSCC) is a prevalent oral cancer.
  • Long non-coding RNA protein kinase cGMP-dependent type I-Antisense RNA 1 (PRKG1-AS1) role in OSCC is under investigation.

Purpose of the Study:

  • To investigate the influence of PRKG1-AS1 on OSCC progression.
  • To assess the prognostic value of PRKG1-AS1 in OSCC patients.

Main Methods:

  • TCGA database analysis for PRKG1-AS1 expression and prognosis.
  • Quantitative real-time PCR (qRT-PCR) for PRKG1-AS1 expression in OSCC cells.
  • Cell Counting Kit-8 (CCK8), colony-forming, and Transwell assays for cell growth, invasion, and migration.
  • Western blotting for epithelial-mesenchymal transition (EMT) markers.

Main Results:

  • PRKG1-AS1 was highly expressed in OSCC tissues and linked to poor outcomes.
  • PRKG1-AS1 expression was elevated in OSCC cell lines (CAL27, SCC-9, SCC-4) compared to normal cells.
  • PRKG1-AS1 deficiency suppressed, while overexpression accelerated, OSCC cell growth, invasion, and migration.
  • PRKG1-AS1 modulated EMT markers, including E-cadherin, N-cadherin, Vimentin, and Snail.

Conclusions:

  • PRKG1-AS1 acts as a facilitator in OSCC cell growth, migration, and invasion.
  • PRKG1-AS1 likely regulates OSCC progression through epithelial-mesenchymal transition (EMT).
Keywords:
epithelial-mesenchymal transitiongrowthinvasionmigrationoral squamous cell carcinomaprotein kinase cGMP-dependent type I-antisense RNA 1

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