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Related Experiment Video

Updated: Jan 2, 2026

A Multimodal Imaging Approach Based on Micro-CT and Fluorescence Molecular Tomography for Longitudinal Assessment of Bleomycin-Induced Lung Fibrosis in Mice
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Microenvironment-Responsive Small-Molecule Probe for Pulmonary Fibrosis Detection.

Ying Dong1,2, Xiao-Rong Li3, Qi Chen4

  • 1State Key Laboratory of Drug Research , Shanghai Institute of Materia Medica, Chinese Academy of Sciences , Shanghai 201203 , China.

Analytical Chemistry
|December 3, 2019
PubMed
Summary

A new probe, PNO1, detects pulmonary fibrosis (PF) by sensing nitric oxide (NO) levels. This noninvasive method shows promise for early PF diagnosis and drug screening.

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Last Updated: Jan 2, 2026

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Area of Science:

  • Biomedical Engineering
  • Medical Diagnostics
  • Molecular Probes

Background:

  • Pulmonary fibrosis (PF) is a severe, progressive lung disease with limited early diagnostic options.
  • Early and noninvasive diagnosis of PF is crucial for improving patient prognosis.
  • Increased nitric oxide (NO) is a characteristic biomarker of the PF microenvironment.

Purpose of the Study:

  • To develop and validate a novel small-molecule probe, PNO1, for the noninvasive detection of pulmonary fibrosis.
  • To assess the efficacy of PNO1 in detecting PF in vivo, in vitro, and ex vivo.
  • To establish PNO1 as a complementary tool for PF diagnosis and anti-PF drug screening.

Main Methods:

  • Synthesis and characterization of the fluorogenic small-molecule probe PNO1.
  • In vivo fluorescence imaging of PNO1 in PF-diseased and control mice lungs.
  • In vitro and ex vivo analysis of PNO1 fluorescence in PF-diseased cells and patient tissues.

Main Results:

  • PNO1 exhibited significantly higher fluorescence (6-fold increase) in the lungs of PF-diseased mice compared to controls.
  • PNO1 successfully detected PF in diseased cells and patient tissues.
  • The probe's fluorescence correlated with the presence and severity of pulmonary fibrosis.

Conclusions:

  • PNO1 is a sensitive and specific fluorogenic probe for detecting pulmonary fibrosis.
  • PNO1 offers a noninvasive and rapid method for PF diagnosis, complementing traditional techniques.
  • PNO1 holds potential for facilitating early disease detection and accelerating anti-PF drug development.