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Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
Published on: May 12, 2023
AAAA-DDDD Quadruple H-Bond-Assisted Ionic Interactions: Robust Bis(guanidinium)/Dicarboxylate Heteroduplexes in Water
Yuren Sun1, Jun Gu1, Hongyu Wang1
1School of Materials Science and Engineering, Center for Supramolecular Chemistry and Catalysis, and Department of Chemistry , Shanghai University , 99 Shang-Da Road , Shanghai 200444 , China.
Abstract:
The use of geminal di(guanidinium) and acridin-9(10H)-one-derived di(carboxylate) derivatives (1a-c and 2a-e, respectively) allows stabilization of heterodimers characterized by high binding affinities in water (maximum ΔG < -7 kcal mol-1, Ka > 105 M-1) as inferred from UV-vis spectroscopic titrations and ITC measurements, therefore rivaling or surpassing the interaction energy between the strongest DNA or RNA triplet pairs. These duplexes are readily accessible and are structurally modifiable, rendering them attractive as building blocks for creating heteroduplex constructs. Incorporating poly(ethylene glycol)-decorated benzyl groups into the dicarboxylate, allows formation of hydrogels in the case of 1b-2c.
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