Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Master Transcription Regulators02:23

Master Transcription Regulators

7.6K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.6K
Master Transcription Regulators02:23

Master Transcription Regulators

2.7K
2.7K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

8.3K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
8.3K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

2.9K
2.9K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

7.1K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
7.1K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

2.4K
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AAK1-mediated phosphorylation of PDLIM5 and Talin1 promotes focal adhesion disassembly to accelerate cell migration.

Nature communications·2026
Same author

Structural basis of the cyclin Y/14-3-3 protein-mediated activation of CDK16.

Nature communications·2026
Same author

Carbene-catalyzed double esterification enables enantioselective conformational self-locking of pillar[5]arenes.

Nature communications·2026
Same author

Lock, relax, load, and shoot: a molecular perspective on Nedd4 regulation.

The FEBS journal·2026
Same author

Paving the Way for CCK2R-Targeted Peptide Receptor Radionuclide Therapy with [<sup>177</sup>Lu]Lu-DOTA-MGS5 in Patients with Small Cell Lung Cancer.

Pharmaceutics·2026
Same author

Amphibian-Derived Peptide Analog TB_KKG6K: A Powerful Drug Candidate Against <i>Candida albicans</i> with Anti-Biofilm Efficacy.

Journal of fungi (Basel, Switzerland)·2026

Related Experiment Video

Updated: Jan 2, 2026

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
14:47

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

10.2K

Modulating FOXO3 transcriptional activity by small, DBD-binding molecules.

Judith Hagenbuchner1, Veronika Obsilova2, Teresa Kaserer3,4,5

  • 1Department of Pediatrics II, Medical University Innsbruck, Innsbruck, Austria.

Elife
|December 3, 2019
PubMed
Summary

Researchers identified small molecules that bind to the FOXO3 DNA-binding domain (DBD), modulating gene transcription and cellular processes. These compounds offer a new strategy for targeting FOXO transcription factors in both normal and cancer cells.

Keywords:
FOXO transcription factorsbiochemistrycancer biologychemical biologydockingdrug targetinghumanmolecular biophysicspharmacophore modellingsmall compoundsstructural biology

More Related Videos

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
09:58

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Published on: June 27, 2020

3.1K
Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

8.9K

Related Experiment Videos

Last Updated: Jan 2, 2026

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
14:47

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

10.2K
Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
09:58

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Published on: June 27, 2020

3.1K
Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

8.9K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Pharmacology

Background:

  • FOXO transcription factors are essential for maintaining cell homeostasis, regulating critical processes like cell death, differentiation, and longevity.
  • Dysregulation of FOXO factors is implicated in various physiological and pathological conditions, including cancer.

Purpose of the Study:

  • To identify small molecules that physically interact with the DNA-binding domain (DBD) of FOXO3.
  • To investigate the functional consequences of this interaction on FOXO3 transcriptional activity and cellular programs.

Main Methods:

  • Combined pharmacophore-modeling-based in silico screening and fluorescence polarization assays to identify interacting compounds.
  • NMR spectroscopy and molecular docking studies to elucidate the mode of interaction between compounds and the FOXO3-DBD.
  • Assays to assess the impact of compounds on FOXO3 target promoter binding and gene transcription in human cancer cells.

Main Results:

  • Identified small molecules, including S9 and its oxalate salt S9OX, that bind to the FOXO3-DBD.
  • Demonstrated that these compounds interfere with FOXO3's ability to bind target promoters and regulate gene transcription.
  • Showed that S9 and S9OX modulate the physiological programs activated by FOXO3 in cancer cells.

Conclusions:

  • The FOXO3-DBD is a druggable target, amenable to modulation by small molecules.
  • The identified compounds provide a structural basis for developing novel therapeutics targeting FOXO-mediated pathways.
  • These findings open avenues for modulating FOXO activity in both normal physiological contexts and malignant diseases.