Fast Affinity Induced Reaction Sensor Based on a Fluorogenic Click Reaction for Quick Detection of Protein Biomarkers

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Immunotechnology

Background:

• Traditional biomarker detection methods, such as ELISA and Western blot, are time-consuming and labor-intensive.
• Existing biosensors often face limitations in generality, specificity, or speed.
• There is a need for faster, more efficient methods for routine biomarker quantification.

Purpose of the Study:

• To introduce and characterize a novel Fast Affinity Induced Reaction Sensor (FAIRS) for rapid biomarker detection.
• To demonstrate the general applicability and specificity of the FAIRS technology.
• To evaluate the performance of FAIRS for quantifying biomarkers in biological samples.

Main Methods:

• Development of FAIRS probes utilizing a sandwich ELISA antibody pair conjugated with fluorogenic click chemicals.
• Leveraging differences in antibody affinity and chemical reaction rates for probe design and optimization.
• Characterization of FAIRS stability, sensitivity, detection range, and response time.
• Application of FAIRS for Interleukin-6 (IL-6) detection in blood serum and cell culture medium.

Main Results:

• FAIRS demonstrated high sensitivity and a wide detection range for IL-6 quantification.
• The sensor operates as a general, one-step detection method with inherent specificity.
• Response times were significantly reduced compared to traditional assays.
• Successful quantification of IL-6 in complex biological matrices like serum and cell culture medium.

Conclusions:

• FAIRS technology provides a fast, specific, and sensitive platform for biomarker detection.
• The one-step nature and broad applicability make FAIRS suitable for various medical and clinical diagnostic applications.
• FAIRS offers a promising alternative to conventional methods, addressing the demand for rapid biomarker analysis.