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Updated: Jan 2, 2026

Quantitative Hardness Measurement by Instrumented AFM-indentation
Published on: November 22, 2016
Gypsum: an environment-friendly, inexpensive and robust height calibration standard at nanometer-scale for atomic
Ana P M Barboza1, Joyce C C Santos2, Elisangela Silva-Pinto3
1Department of Physics, Universidade Federal de Ouro Preto, 35400-000, Ouro Preto, MG, Brazil.
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Gypsum is an Earth-abundant mineral with enormous applications in agriculture and civil engineering. Here, we show it is also an excellent height calibration standard alternative for atomic force microscopy (AFM). Using plain water as etchant, gypsum flakes readily review 0.75 nm tall terraces which are easy to image (lateral dimensions from tens to hundreds of nanometers) and robust against time in ambient conditions. Therefore, the present work demonstrates a new height standard alternative which is easily-available for all AFM microscopists around the world.

