Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

6.4K
Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
6.4K
Nucleic Acid Structure01:25

Nucleic Acid Structure

8.3K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
8.3K
Nucleic Acids02:43

Nucleic Acids

49.3K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
49.3K
Nucleic acids02:43

Nucleic acids

187.9K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
187.9K
RNA Structure01:19

RNA Structure

6.7K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
6.7K
RNA Structure01:23

RNA Structure

78.6K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
78.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatially Adaptive Varying Correlation Analysis for Multimodal Neuroimaging Data.

IEEE transactions on medical imaging·2018
Same author

Superconductivity in FeSe: The Role of Nematic Order.

Physical review letters·2018
Same author

Suppressed expression of Cbl-b by NF-κB mediates icotinib resistance in EGFR-mutant non-small-cell lung cancer.

Cell biology international·2018
Same author

The independence of and associations among apoptosis, autophagy, and necrosis.

Signal transduction and targeted therapy·2018
Same author

Tyrosine kinase inhibitor-induced IL-6/STAT3 activation decreases sensitivity of EGFR-mutant non-small cell lung cancer to icotinib.

Cell biology international·2018
Same author

Experimental Study on Productivity Performance of Household Combined Thermal Power and Biogas System in Northwest China.

BioMed research international·2018

Related Experiment Video

Updated: Jan 2, 2026

Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET
12:07

Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET

Published on: October 9, 2021

3.7K

ATP binds nucleic-acid-binding domains beyond RRM fold.

Yuan He1, Jian Kang1, Liangzhong Lim1

  • 1Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, 119260, Singapore.

Biochemical and Biophysical Research Communications
|December 4, 2019
PubMed
Summary

Adenosine triphosphate (ATP) at millimolar concentrations controls protein homeostasis and phase separation. New findings show ATP also binds nucleic acid-binding proteins, suggesting broader cellular roles.

Keywords:
Acidic domain (AcD)Adenosine triphosphate (ATP)Molecular dockingNMR spectroscopyNon-canonical RNA-Binding domainSYNCRIP (hnRNP Q)

More Related Videos

CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

7.5K
mRNA Interactome Capture from Plant Protoplasts
12:29

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

9.5K

Related Experiment Videos

Last Updated: Jan 2, 2026

Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET
12:07

Chemical Modification of the Tryptophan Residue in a Recombinant Ca2+-ATPase N-domain for Studying Tryptophan-ANS FRET

Published on: October 9, 2021

3.7K
CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

7.5K
mRNA Interactome Capture from Plant Protoplasts
12:29

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

9.5K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Cells maintain high adenosine triphosphate (ATP) concentrations (2-12 mM), exceeding known functional requirements.
  • ATP's role as a hydrotrope controlling protein homeostasis above 5 mM has been recently uncovered.
  • ATP's specific binding mediates liquid-liquid phase separation and inhibits RRM domain fibrillation.

Purpose of the Study:

  • To investigate if ATP interacts with nucleic acid-binding proteins lacking the RRM fold.
  • To characterize the interaction between ATP and the SYNCRIP acidic domain (AcD).

Main Methods:

  • Nuclear Magnetic Resonance (NMR) studies were employed.
  • Characterization of ATP's interaction with the SYNCRIP acidic domain (AcD).

Main Results:

  • ATP binds to the SYNCRIP acidic domain (AcD) at physiologically relevant concentrations.
  • The binding affinity determinants resemble those of protein-nucleic acid interactions.
  • This suggests ATP may interact with most nucleic acid-binding proteins at millimolar concentrations.

Conclusions:

  • ATP possesses functions beyond energy provision and hydrotropic control.
  • ATP's specific binding to proteins, including non-RRM domains, indicates a broader regulatory role in cellular processes.
  • This discovery redefines our understanding of ATP's cellular functions and interactions.