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Related Concept Videos

Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
12.8K

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Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models
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Molecular Imaging with Reporter Genes: Has Its Promise Been Delivered?

Inna Serganova1, Ronald G Blasberg2,3,4

  • 1Department of Neurology, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, New York.

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Reporter gene systems visualize gene activity. This review focuses on radionuclide-based systems, examining their development and potential for clinical translation in imaging.

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Medical Imaging

Background:

  • Reporter gene systems, developed in the 1980s, measure enzyme activity as a surrogate for promoter-driven transcription.
  • Initial applications focused on visualizing specific promoter activity with high sensitivity.
  • Reporter systems are broadly categorized into constitutively expressed and inducible types.

Purpose of the Study:

  • To review the evolution of reporter systems, including fluorescent, bioluminescent, and radionuclide-based approaches.
  • To concentrate on the development and applications of radionuclide-based reporter systems.
  • To assess the clinical translation potential of reporter gene imaging.

Main Methods:

  • Review of historical development of reporter gene systems.
  • Analysis of fluorescent, bioluminescent, and radionuclide-based reporter technologies.
  • Discussion of applications in molecular biology and medical imaging.

Main Results:

  • Reporter systems have evolved significantly since their inception.
  • Radionuclide-based systems offer unique advantages for in vivo imaging.
  • The full promise of reporter gene imaging, particularly with radionuclide-based systems, is still under evaluation.

Conclusions:

  • Further development is needed to realize the full potential of radionuclide-based reporter systems.
  • Successful clinical translation requires addressing specific technical and regulatory challenges.
  • Reporter gene imaging holds promise for advancing diagnostics and therapeutics.