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CRISPR-cas9: a powerful tool towards precision medicine in cancer treatment

Hui Xing1,2, Ling-Hua Meng3,4

  • 1Division of Anti-tumor Pharmacology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.

Acta Pharmacologica Sinica
|December 4, 2019
PubMed

Insights

CRISPR-cas9 screening advances precision medicine by identifying cancer drug targets and resistance mechanisms. This powerful gene editing tool aids in overcoming tumor heterogeneity and improving patient outcomes.

Area of Science:

  • Oncology
  • Genetics
  • Biotechnology

Background:

  • Cancer exhibits significant molecular heterogeneity, contributing to treatment resistance and recurrence.
  • Understanding tumorigenesis and drug resistance is crucial for developing effective precision medicine strategies.
  • CRISPR-cas9 gene editing offers powerful capabilities for high-throughput screening in cancer research.

Purpose of the Study:

  • To review the progress of CRISPR-cas9-based unbiased screening in precision medicine.
  • To highlight the role of CRISPR-cas9 in identifying novel drug targets and biomarkers.
  • To elucidate mechanisms underlying cancer drug resistance using CRISPR-cas9 screening.

Main Methods:

  • CRISPR-cas9-based high-throughput screening.
  • Unbiased screening approaches for gene identification.
  • Analysis of tumorigenesis and drug resistance mechanisms.

Main Results:

  • CRISPR-cas9 screening facilitates the discovery of tumor-driving and synergistic lethal genes.
  • Identification of potential new drug targets and predictive biomarkers for cancer therapy.
  • Elucidation of key molecular mechanisms contributing to acquired drug resistance.

Conclusions:

  • CRISPR-cas9 screening is a transformative tool in precision oncology.
  • It holds significant promise for overcoming drug resistance and improving therapeutic strategies.
  • Further research is needed to address current challenges and optimize applications.
Keywords:
CRISPR-cas9biomarkerdrug resistancedrug targetprecision medicinesynergistic lethality

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