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An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
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Interactions Between MAIT Cells and Dendritic Cells

Mariolina Salio1, Vincenzo Cerundolo2

  • 1MRC Human Immunology Unit, MRC Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK. Mariolina.salio@imm.ox.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|December 4, 2019
PubMed

Mucosal-associated invariant T (MAIT) cells recognize intermediates of the vitamin B2 biosynthetic pathway present in a variety of bacteria, presented by the monomorphic MR1 molecules. Because of their central role in shaping adaptive immunity through interaction with dendritic cells (DCs) and B cells, their manipulation can be of translational relevance. We describe a method to routinely isolate and maintain MAIT cells from peripheral blood and to investigate their activity using DC as targets.

Keywords:
CD40LMAITMR1 dendritic cells

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