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Carnosine treatment during human semen processing by discontinuous density gradient.

Luana Nayara Gallego Adami1, Bruna Tuani de Lima1, Rhayza Roberta Andretta1

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Carnosine supplementation during human semen processing improved sperm mitochondrial activity and beat-cross frequency (BCF). Both 20 mM and 50 mM carnosine enhanced BCF, while 50 mM carnosine notably boosted mitochondrial activity.

Keywords:
antioxidantsfree radicalsmitochondriaoxidative stressspermatozoa

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Area of Science:

  • Reproductive Biology
  • Biochemistry

Background:

  • Semen processing is crucial for assisted reproductive technologies.
  • Optimizing sperm quality during processing is an ongoing area of research.
  • Carnosine is an endogenous antioxidant with potential protective effects on sperm.

Purpose of the Study:

  • To investigate the impact of varying carnosine concentrations on human sperm quality during density gradient centrifugation.
  • To assess the effects of carnosine on sperm kinetics and mitochondrial function.

Main Methods:

  • Human semen samples (n=34) were processed using discontinuous density gradient centrifugation.
  • Supplementation with 20 mM or 50 mM carnosine was applied to the Percoll layers.
  • Sperm samples were washed and analyzed for motility parameters and mitochondrial activity.
  • Statistical analysis employed general linear models or Friedman tests.

Main Results:

  • Sperm motility parameters (percentage motile, progressive motility, velocities) improved post-processing, independent of carnosine.
  • Both 20 mM and 50 mM carnosine concentrations significantly increased beat-cross frequency (BCF) compared to pre-processing.
  • 50 mM carnosine supplementation demonstrated a significant improvement in sperm mitochondrial activity versus controls.

Conclusions:

  • Carnosine supplementation during semen processing positively influences sperm mitochondrial activity.
  • Carnosine enhances beat-cross frequency (BCF), a measure of sperm flagellar beating.
  • The findings suggest carnosine as a beneficial additive for improving sperm quality in processing.